Bovine Prostaglandin-H2 D-isomerase, PTGDS ELISA Kit | MyBioSource MBS942806 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Bovine Prostaglandin-H2 D-isomerase, PTGDS ELISA Kit

catalog :

MBS942806

quantity :

48-Strip-Wells

price :

565 USD

product information

catalog number :

MBS942806

products type :

ELISA Kit

products full name :

Bovine Prostaglandin-H2 D-isomerase, PTGDS ELISA Kit

products short name :

prostaglandin D2 synthase 21kDa (brain)

products name syn :

Bovine Prostaglandin-H2 D-isomerase (PTGDS) ELISA kit; RP11-229P13.6; L-PGDS; LPGDS; PDS; PGD2; PGDS; PGDS2; PGD2 synthase; beta-trace protein; glutathione-independent PGD synthase; lipocalin-type prostaglandin D synthase; prostaglandin D synthase; prostagla; prostaglandin D2 synthase 21kDa (brain)

other names :

prostaglandin-H2 D-isomerase; Prostaglandin-H2 D-isomerase; prostaglandin-H2 D-isomerase; PGDS; PGDS2; PGD2 synthase; prostaglandin-D2 synthase; glutathione-independent PGD synthase; glutathione-independent PGD synthetase; lipocalin-type prostaglandin-D synthase; prostaglandin D2 synthase 21kDa (brain); Glutathione-independent PGD synthase; Lipocalin-type prostaglandin-D synthase; Prostaglandin-D2 synthase; PGD2 synthase; PGDS; PGDS2

products gene name :

PTGDS

other gene names :

PTGDS; PTGDS; PGD2 synthase; PGDS; PGDS2

uniprot entry name :

PTGDS_BOVIN

reactivity :

Bovine

sequence length :

191

specificity :

This assay has high sensitivity and excellent specificity for detection of bovine PTGDS. No significant cross-reactivity or interference between bovine PTGDS and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 93.75 pg/ml -6000 pg/ml. Sensitivity: The minimum detectable dose of bovine PTGDS is typically less than 46.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest bovine PTGDS concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTGDS. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated PTGDS. The competitive inhibition reaction is launched between with HRP-conjugated PTGDS and PTGDS in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of PTGDS in the sample. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

27807521

ncbi acc num :

NP_777216.1

ncbi gb acc num :

NM_174791.4

uniprot acc num :

O02853

ncbi mol weight :

21,229 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (84234); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198559)

uniprot summary :

Function: Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system . Catalytic activity: (5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E,15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate.1 PublicationManual assertion based on experiment in:Ref.1

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150