Human Protein Wnt-9a, WNT9A ELISA Kit | MyBioSource MBS942617 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Protein Wnt-9a, WNT9A ELISA Kit

catalog :

MBS942617

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS942617

products type :

ELISA Kit

products full name :

Human Protein Wnt-9a, WNT9A ELISA Kit

products short name :

wingless-type MMTV integration site family, member 9A

products name syn :

Human Protein Wnt-9a (WNT9A) ELISA kit; MGC138165; MGC141991; WNT14; wingless-type MMTV integration site family; member 14; wingless-type MMTV integration site family; member 9A

other names :

protein Wnt-9a; Protein Wnt-9a; protein Wnt-9a; wingless-type MMTV integration site family, member 14; wingless-type MMTV integration site family, member 9A; Protein Wnt-14

products gene name :

WNT9A

other gene names :

WNT9A; WNT9A; WNT14; WNT14

uniprot entry name :

WNT9A_HUMAN

reactivity :

Human

sequence length :

365

specificity :

This assay has high sensitivity and excellent specificity for detection of human WNT9A. No significant cross-reactivity or interference between human WNT9A and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human WNT9A is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT9A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT9A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT9A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT9A bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

15082261

ncbi acc num :

NP_003386.1

ncbi gb acc num :

NM_003395.2

uniprot acc num :

O14904

ncbi mol weight :

40,320 Da

ncbi pathways :

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Class B/2 (Secretin Family Receptors) Pathway (106378); GPCR Ligand Binding Pathway (161020); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83063); Hedgehog Signaling Pathway (474); Melanogenesis Pathway (83092); Melanogenesis Pathway (504)

ncbi summary :

The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is expressed in gastric cancer cell lines. The protein encoded by this gene shows 75% amino acid identity to chicken Wnt14, which has been shown to play a central role in initiating synovial joint formation in the chick limb. This gene is clustered with another family member, WNT3A, in the chromosome 1q42 region. [provided by RefSeq, Jul 2008]

uniprot summary :

WNT9A: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1q42. Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region. Molecular Function: frizzled binding. Biological Process: embryonic forelimb morphogenesis; Wnt receptor signaling pathway; cell fate commitment; multicellular organismal development; mitotic cell cycle checkpoint; negative regulation of chondrocyte differentiation; Wnt receptor signaling pathway through beta-catenin; negative regulation of caspase activity; embryonic skeletal morphogenesis; neuron differentiation; negative regulation of cell proliferation; cell-cell signaling; positive regulation of smoothened signaling pathway; positive regulation of cell differentiation

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage