ELISA/assay

General Chondroitin sulfate ELISA Kit

MBS9426026

96 Tests

625 USD

ELISA Kit

General Chondroitin sulfate ELISA Kit

[Chondroitin sulfate]

[Chondroitin sulfate; CS]

[CS]

General

This assay recognizes recombinant and natural General Chondroitin sulfate. No significant cross-reactivity or interference was observed.

Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Assay Type: Quantitative Competitive. Detection Range: 0.78-50.0 ng/mL.

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of general Chondroitin sulfate concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated w~h an antibody specific to Chondroitin sulfate, During the reaction, Chondro~in sulfate in the sample or standard competes with a fixed amount of biotin-labeled Chondro~in sulfate for sites on a pre-coated Monoclonal antibody specific to Chondro~in sulfate. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm :I: 2 nm. The concentration of Chondro~in sulfate in the samples is then determined by comparing the 0.0. of the samples to the standard curve.

96 Tests

625 USD