Mouse Thrombospondin-1 ELISA Kit | MyBioSource MBS9425883 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Thrombospondin-1 ELISA Kit

catalog :

MBS9425883

quantity :

96 Tests

price :

495 USD

product information

catalog number :

MBS9425883

products type :

ELISA Kit

products full name :

Mouse Thrombospondin-1 ELISA Kit

products short name :

[Thrombospondin-1]

products name syn :

[Thrombospondin-1; Thbs1; Tsp1]

other names :

[Thrombospondin-1; Thrombospondin-1; thrombospondin-1; thrombospondin 1]

products gene name :

[Thbs1]

other gene names :

[Thbs1; Thbs1; TSP1; TSP-1; tbsp1; Thbs-1; Tsp1]

reactivity :

Mouse

specificity :

This assay recognizes recombinant and natural mouse Thrombospondin-1. No significant cross-reactivity or interference was observed.

storage stability :

Store at 4 degree C

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Detection Range: 7.80 ng/mL -500 ng/mL. Sensitivity: 4.00 ng/mL

products description :

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of mouse Thrombospondin-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Principle of the Assay: The microtrter plate provided in this krt has been pre-coated with an antibody specific to Thrombospondin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Thrombospondin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Thrombospondin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Thrombospondin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

549134

ncbi acc num :

P35441.1

uniprot acc num :

P35441

ncbi mol weight :

129,647 Da

ncbi pathways :

Bladder Cancer Pathway (83308); Bladder Cancer Pathway (527); ECM-receptor Interaction Pathway (83265); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (1323909); Focal Adhesion Pathway (198353); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478); Hemostasis Pathway (1323559); Inflammatory Response Pathway (198280)

ncbi summary :

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

uniprot summary :

THBS1: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties. Belongs to the thrombospondin family. Protein type: Inhibitor; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 2 E5 2 59.34 cM. Cellular Component: cell surface; cytoplasm; endoplasmic reticulum; external side of plasma membrane; extracellular matrix; extracellular space; fibrinogen complex; sarcoplasmic reticulum; secretory granule. Molecular Function: extracellular matrix binding; fibroblast growth factor binding; fibronectin binding; heparin binding; integrin binding; laminin binding; low-density lipoprotein binding; phosphatidylserine binding; protein binding; transforming growth factor beta binding. Biological Process: activation of MAPK activity; behavioral response to pain; blood vessel morphogenesis; cell cycle arrest; cell migration; engulfment of apoptotic cell; inflammatory response; negative regulation of angiogenesis; negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II; negative regulation of apoptosis; negative regulation of blood vessel endothelial cell migration; negative regulation of caspase activity; negative regulation of cell-matrix adhesion; negative regulation of dendritic cell antigen processing and presentation; negative regulation of endothelial cell proliferation; negative regulation of fibrinolysis; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of interleukin-12 production; peptide cross-linking; positive regulation of angiogenesis; positive regulation of blood coagulation; positive regulation of blood vessel endothelial cell migration; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of chemotaxis; positive regulation of macrophage activation; positive regulation of phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of smooth muscle cell proliferation; positive regulation of transforming growth factor beta receptor signaling pathway; positive regulation of translation; positive regulation of tumor necrosis factor biosynthetic process; regulation of cGMP metabolic process; response to calcium ion; response to glucose stimulus; response to magnesium ion; response to mechanical stimulus; sprouting angiogenesis

size1 :

96 Tests

price1 :

495 USD