catalog number :
MBS9425711
products type :
ELISA Kit
products full name :
Mouse Programmed cell death protein 1 ELISA Kit
products short name :
[Programmed cell death protein 1]
products name syn :
[Programmed cell death protein 1; Protein PD-1; mPD-1; Pdcd1; Pd1; CD279]
other names :
[programmed cell death protein 1; Programmed cell death protein 1; programmed cell death protein 1; programmed cell death 1; CD_antigen: CD279]
products gene name :
[Pdcd1]
other gene names :
[Pdcd1; Pdcd1; PD-1; Pdc1; Ly101; Pd1; Protein PD-1; mPD-1]
specificity :
This assay recognizes recombinant and natural mousemPD-1. No significant cross-reactivity or interference was observed.
storage stability :
Store at 4 degree C
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates and cell culture supernates and other biological fluids. Detection Range: 1.15ng/ml-10.0ng/ml.
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to mPD-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific formPD-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain mPD-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of mPD-1 in the samples is then determined by comparing the 0.0. of the samples to the standard curve.
ncbi acc num :
NP_032824.1
ncbi gb acc num :
NM_008798.2
ncbi mol weight :
31,842 Da
ncbi pathways :
Adaptive Immune System Pathway (1110669); Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); Costimulation By The CD28 Family Pathway (1110675); Immune System Pathway (1110668); PD-1 Signaling Pathway (1110680); T Cell Receptor Signaling Pathway (83277); T Cell Receptor Signaling Pathway (491)
uniprot summary :
PDCD1: a single-pass type I membrane protein. A negative regulator of activated T cells, is upregulated in human and mouse 'exhausted' T cells during persistent viral infection. A marker of angioimmunoblastic T-cell lymphoma and germinal center associated T cells. Protein type: Apoptosis; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1 1 D. Cellular Component: external side of plasma membrane; plasma membrane. Molecular Function: protein binding. Biological Process: negative regulation of apoptosis; negative regulation of tolerance induction; positive regulation of apoptosis