ELISA/assay

Rat Protein CYR61 ELISA Kit

MBS9425693

96 Tests

575 USD

ELISA Kit

Rat Protein CYR61 ELISA Kit

[CYR61]

[Protein CYR61; CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Cyr61; Ccn1; Igfbp10]

[protein CYR61; Protein CYR61; protein CYR61; cysteine-rich, angiogenic inducer, 61; CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10]

[CYR61]

[Cyr61; Cyr61; Ccn1; Igfbp10; IBP-10; IGF-binding protein 10; IGFBP-10]

Rat

This assay recognizes recombinant and natural ratProtein CYR61. No significant cross-reactivity or interference was observed. Note?Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between ratProtein CYR61and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids. Detection Range: 0.31ng/mL -20.0ng/mL.

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination ofratProtein CYR61concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein CYR61. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forProtein CYR61 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein CYR61, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Protein CYR61 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

31542447

NP_112617.2

NM_031327.2

Q9ES72

41,687 Da

Hypertrophy Model Pathway (198527)

an extracellular heparin binding protein involved in supporting smooth muscle cell adhesion, promoting cell migration, and enhancing growth factor-stimulated mitogenesis [RGD, Feb 2006]

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5 (). CYR61-mediated gene regulation is dependent on heparin-binding (). Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1 (). Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3 ().

96 Tests

575 USD