catalog number :
MBS9425646
products type :
ELISA Kit
products full name :
General Thyroxine ELISA Kit
products short name :
[Thyroxine]
products name syn :
[Thyroxine; T4]
products gene name :
[T4]
specificity :
This assay recognizes recombinant and natural General Thyroxine No significant cross-reactivity or interference was observed.
storage stability :
Store at 4 degree C
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Assay Type: Competitive. Detection Range: 0.19-12.5ng/mL.
products description :
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroxine, During the reaction, Thyroxinein the sample or standard competes with a fixed amount of biotin-labeled Thyroxine for sites on a pre-coated Monoclonal antibody specific to Thyroxine. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Thyroxine in the samples is then determined by comparing the 0.0. of the samples to the standard curve.