catalog number :
MBS942562
products type :
ELISA Kit
products full name :
Rat Apolipoprotein M, APOM ELISA Kit
products short name :
[apolipoprotein M]
products name syn :
[Rat Apolipoprotein M (APOM) ELISA kit; DADB-127H9.5; G3a; HSPC336; MGC22400; NG20; NG20-like protein; alternative name: G3a; NG20; apolipoprotein M]
other names :
[apolipoprotein M; Apolipoprotein M; apolipoprotein M; apo-M; protein Px; apolipoprotein M; Protein Px]
products gene name :
[APOM]
other gene names :
[Apom; Apom; Apo-M; ApoM]
uniprot entry name :
APOM_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat APOM. No significant cross-reactivity or interference between rat APOM and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: The minimum detectable dose of rat APOM is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APOM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APOM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APOM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOM bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_062246.1
ncbi gb acc num :
NM_019373.2
ncbi mol weight :
21,513 Da
ncbi summary :
human homolog is a lipoprotein associated with high density lipoprotein (HDL) particles [RGD, Feb 2006]
uniprot summary :
APOM: Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid. Plasma protein. Expressed in liver and kidney. Belongs to the calycin superfamily. Lipocalin family. Highly divergent. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular space; extracellular region. Molecular Function: lipid transporter activity; antioxidant activity; phospholipid binding. Biological Process: reverse cholesterol transport; response to glucose stimulus; cholesterol efflux; lipoprotein metabolic process
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells