Human Serine protease inhibitor Kazal-type 5, SPINK5 ELISA Kit | MyBioSource MBS942561 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Serine protease inhibitor Kazal-type 5, SPINK5 ELISA Kit

catalog :

MBS942561

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS942561

products type :

ELISA Kit

products full name :

Human Serine protease inhibitor Kazal-type 5, SPINK5 ELISA Kit

products short name :

serine peptidase inhibitor, Kazal type 5

products name syn :

Human Serine protease inhibitor Kazal-type 5 (SPINK5) ELISA kit; DKFZp686K19184; FLJ21544; FLJ97536; FLJ97596; FLJ99794; LEKTI; LETKI; NETS; NS; VAKTI; OTTHUMP00000165952; lymphoepithelial Kazal-type-related inhibitor; serine protease inhibitor; Kazal type 5; serine peptidase inhibitor; Kazal type 5

other names :

serine protease inhibitor Kazal-type 5 isoform a; Serine protease inhibitor Kazal-type 5; Lympho-epithelial Kazal-type-related inhibitor; LEKTI

products gene name :

SPINK5

other gene names :

SPINK5; LEKTI

uniprot entry name :

ISK5_HUMAN

reactivity :

Human

sequence length :

1094

specificity :

This assay has high sensitivity and excellent specificity for detection of human SPINK5. No significant cross-reactivity or interference between human SPINK5 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: 15.6 pg /ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPINK5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPINK5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPINK5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPINK5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

189163501

ncbi acc num :

NP_001121170.1

ncbi gb acc num :

NM_001127698.1

uniprot acc num :

Q9NQ38

ncbi mol weight :

120,714 Da

ncbi summary :

This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The inhibitor may play a role in skin and hair morphogenesis and anti-inflammatory and/or antimicrobial protection of mucous epithelia. Mutations may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

SPINK5: Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense- activating and desquamation-involved proteases. Inhibits KLK5, it s major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin. Defects in SPINK5 are the cause of Netherton syndrome (NETH). NETH is an autosomal recessive congenital ichthyosis associated with hair shaft abnormalities and anomalies of the immune system. Typical features are ichthyosis linearis circumflexa, ichthyosiform erythroderma, trichorrhexis invaginata (bamboo hair), atopic dermatitis, and hayfever. High postnatal mortality is due to failure to thrive, infections and hypernatremic dehydration. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Inhibitor. Chromosomal Location of Human Ortholog: 5q32. Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; extracellular region; cell cortex; cytosol. Molecular Function: serine-type endopeptidase inhibitor activity. Biological Process: extracellular matrix organization and biogenesis; negative regulation of angiogenesis; epithelial cell differentiation; negative regulation of immune response; hair cell differentiation; epidermal cell differentiation; regulation of T cell differentiation; anagen. Disease: Ige Responsiveness, Atopic; Netherton Syndrome

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage