ELISA/assay

Human Neutrophil elastase ELISA Kit

MBS9425309

96 Tests

575 USD

ELISA Kit

Human Neutrophil elastase ELISA Kit

[Neutrophil elastase]

[Neutrophil elastase; Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase; ELANE; ELA2; 3.4.21.37]

[neutrophil elastase preproprotein; Neutrophil elastase; neutrophil elastase; elastase, neutrophil expressed; Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase]

[ELANE]

[ELANE; ELANE; GE; NE; HLE; HNE; ELA2; SCN1; PMN-E; ELA2; HLE]

Human

This assay recognizes recombinant and natural human Neutrophil elastase. No significant cross-reactivity or interference was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between human Neutrophil elastase and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Detection Range: 78.1 /mL -5000 pg/mL.

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Neutrophil elastase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil elastase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin- conjugated polyclonal antibody preparation specific for Neutrophil elastase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neutrophil elastase, biotin-conjugated antibody and enzyme- conjugated Avidin will exhibit a change in color. The enzyme- substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Neutrophil elastase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4503549

NP_001963.1

NM_001972.3

P08246

28,518 Da

Activation Of Matrix Metalloproteinases Pathway (576264); C-MYB Transcription Factor Network Pathway (138073); Degradation Of Collagen Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Systemic Lupus Erythematosus Pathway (83122); Systemic Lupus Erythematosus Pathway (534); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987); Amb2 Integrin Signaling Pathway (137945)

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]

ELANE: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Defects in ELANE are a cause of cyclic haematopoiesis (CH); also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1). SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Belongs to the peptidase S1 family. Elastase subfamily. Protein type: Cell cycle regulation; Cell surface; EC 3.4.21.37; Motility/polarity/chemotaxis; Protease. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; secretory granule; transcriptional repressor complex. Molecular Function: cytokine binding; endopeptidase activity; heparin binding; peptidase activity; protease binding; protein binding; serine-type endopeptidase activity. Biological Process: acute inflammatory response to antigenic stimulus; antimicrobial humoral response; biosynthetic process of antibacterial peptides active against Gram-negative bacteria; defense response to bacterium; extracellular matrix disassembly; negative regulation of chemokine biosynthetic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; neutrophil degranulation; phagocytosis; positive regulation of interleukin-8 biosynthetic process; positive regulation of smooth muscle cell proliferation; proteolysis; response to UV. Disease: Cyclic Neutropenia; Neutropenia, Severe Congenital, 1, Autosomal Dominant

96 Tests

575 USD