catalog number :
MBS9424861
products type :
ELISA Kit
products full name :
Mouse IgE ELISA KIT
products short name :
[IgE]
products name syn :
[Mouse IgE]
products gene name :
[IgE]
storage stability :
Store at 4 degree C
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Cell culture supernates, serum, and plasma. Assay Type: Quantitative Sandwich. Detection Range: 312.5pglml- 20000 pg/ml. Sensitivity: 156pglmL.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme Immunoassay technique. A monoclonal antibody specific for IgE has been pre-coated onto a microplate. Standard, control or sample and the working solution of Biotin-Conjugate are p,petted into the wells. Following incubation and wash steps, any IgE present is bound by the immobilized antibody and the detection anlibody specific for IgE 'S binds to the combination of capture antibody- IgE In sample Following a wash to remove any unbound combination. and enzyme conjugate IS added to the wells Following incubation and wash steps a substrate IS added. A coloured product is formed in proporlion to the amount of IgE present In the sample The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve IS prepared from seven IgE standard dilutions and IgE sample concentration determined. Background/Introduction: There are five classes of mammalian immunoglobulins: IgA. IgD. IgE, IgG. and IgM. In mice. the IgG class is further divided into four subclasses' IgG1. IgG2a/lgG2c (strain specific). IgG2b. and IgG3.The general immunoglobulin structure is described as two heavy and two light chain (H2L2) polypeptides linked together by disu~ide bridges of cysteine residues. Mouse IgE. also known as reaginic antibody. exists in serum as a 4-chain monomer of 185-200 kDa. Like IgM. the IgE monomers contain a fourth constant region domain. Serum concentrations of IgE are reported to be >10 ug/ml. IgE is cyolytic for mast cells. where it binds to the Fc[1R 1 receptor and is able to mediate the response of granular and lipid mediators of inflammation. including the PCA reaction. The IgE class sWitch is induced by IL-4 as part of the Th2 response.