ELISA/assay

Mouse MIP-2 ELISA KIT

MBS9424852

96 Tests

450 USD

ELISA Kit

Mouse MIP-2 ELISA KIT

[MIP-2]

[Mouse MIP-2]

[macrophage inflammatory protein 2, MIP-2 {N-terminal}]

[MIP-2]

Mouse

This assay recognizes both natural and recombinant mouse MIP-2. The factors listed below were prepared at 50nglml in Standard Isample Diluent and assayed for cross-reactivity and no significanl cross-reactivily or interference was observed.

Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Samples: Cell culture supernates, serum, and plasma. Assay Type: Quantitative Sandwich. Detection Range: 15.6 pg/ml - 1000 pglml. Sensitivity: 7pg/mL.

Principle of the Assay: This assay employs Ihe quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for MIP-2 has been pre-coated onto a microplate. Standards and samples are pipelted into the wells and any MIP-2 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for MIP-2 is added to the wells and binds to the combination of capture antibody-MIP-2 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of MIP-2 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven MIP-2 standard dilutions and MIP-2 sample concentration determined. Background/Introduction: Mouse macrophage inflammatory protein-2 (MIP-2), also known as CXCL2. was originally identified as a heparin-binding protein secreted by an LPS-stimulated mouse macrophage cell line (1). A cDNA clone encoding the protein was isolated from this cell line and characterized (2). Based on its protein and DNA sequences. mouse MIP-2 was classified as a member of the alpha (CXC) chemokine family of inflammatory and immunoregulatory cytokines (3). Mouse MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature mouse MIP-2. The protein sequence of mouse MIP-2 shows approximately 63% identity to that of mouse KC. another mouse alpha chemokine. Mouse MIP-2 is also 60% identical to human GRO and GRO (2). Based on these protein sequence similarities, it is likely that mouse KC and MIP-2 are homologs of human GRO, and chemokines. Since chemokines with protein sequence homology to human IL-S have not been identified in mice, it has been suggested that the mouse KC and MIP-2 are functional homologs of human IL-8 in mice (3. 4). A putative mouse homolog of the human IL-S receptor beta (IL-8 R ) has also been cloned. This receptor shows 71% identity to human IL-8 Rand 68% identity to human IL-8 R . Both mouse KC and MIP-2 bind mouse IL-8 R with high affinity (5). Like human IL-8, mouse MIP-2 exhibits potent neutrophil chemotactic activity and may be a key mediator of neutrophil recruitment in response to tissue injury and infection (3, 4). Increased MIP-2 expression has been found to be associated with neutrophil influx in various inftammatory conditions (6 - 10).

298582

AAB25756.1

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

96 Tests

450 USD