product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Dynein heavy chain 12, axonemal, DNAH12 ELISA Kit
catalog :
MBS942482
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS942482
products type :
ELISA Kit
products full name :
Human Dynein heavy chain 12, axonemal, DNAH12 ELISA Kit
products short name :
dynein, axonemal, heavy chain 12
products name syn :
Human Dynein heavy chain 12; axonemal (DNAH12) ELISA kit; hCG_1779312; DHC3; DLP12; DNAH12L; DNAH7L; DNAHC12; DNAHC3; DNHD2; FLJ40427; FLJ44290; HL-19; HL19; hdhc3; Dynein; heavy chain-5; axonemal dynein heavy chain isotype3; dynein heavy chain 12; dynein hea; dynein; axonemal; heavy chain 12
other names :
dynein heavy chain 12, axonemal isoform 2; Dynein heavy chain 12, axonemal; dynein heavy chain 12, axonemal; dynein, heavy chain-5; ciliary dynein heavy chain 12; axonemal beta dynein heavy chain 12; axonemal dynein heavy chain isotype3; dynein, axonemal, heavy polypeptide 12; dynein heavy chain domain-containing protein 2; dynein, axonemal, heavy chain 12; Axonemal beta dynein heavy chain 12; Axonemal dynein heavy chain 12-like protein; Axonemal dynein heavy chain 7-like protein; Ciliary dynein heavy chain 12; Dynein heavy chain 7-like, axonemal; Dynein heavy chain domain-containing protein 2
products gene name :
DNAH12
other gene names :
DNAH12; DNAH12; DHC3; HL19; DLP12; DNHD2; HDHC3; HL-19; DNAH7L; DNAHC3; DNAH12L; DNAHC12; DHC3; DLP12; DNAH12L; DNAH7L; DNAHC3; DNHD2; HDHC3; HL19
uniprot entry name :
DYH12_HUMAN
reactivity :
Human
sequence length :
457
specificity :
This assay has high sensitivity and excellent specificity for detection of Human DNAH12. No significant cross-reactivity or interference between Human DNAH12 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 12.5 pg/ml-800 pg/ml. Sensitivity: 3.12 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Ubiquitin. Protein Biological Process 3: Ubl conjugation pathway
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DNAH12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DNAH12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNAH12 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNAH12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
89274173
ncbi acc num :
NP_940966.2
ncbi gb acc num :
NM_198564.3
uniprot acc num :
Q6ZR08
ncbi mol weight :
356,942 Da
uniprot summary :
DNAHC12: Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly. Belongs to the dynein heavy chain family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 3p14.3. Cellular Component: dynein complex; microtubule; cytoplasm; cilium. Molecular Function: ATPase activity; microtubule motor activity; ATP binding. Biological Process: metabolic process; microtubule-based movement
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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