ELISA/assay

Rat thyroxine, T4 ELISA Kit

MBS9424770

96 Tests

625 USD

ELISA Kit

Rat thyroxine, T4 ELISA Kit

[thyroxine, T4]

[Thyroxine; T4]

[T4]

Rat

This assay has high sensitivity and excellent specificity for detection of rat T4. No significant cross-reactivity or interference between rat T4 and analogues was observed.

Store at 4 degree C

Samples: Serum, Plasma, Cell Culture Supernates, Tissue Homogenates, Cell Lysates. Assay Type: Competitive. Detection Range: 20 ng/ml-320 ng/ml. Sensitivity: 20 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T4. A competitive inhibition reaction is launched between T4 (Standards or samples) and Biotin-conjugated T4 with the pre-coated antibody specific for T4. The more amount of T4 in samples, the less antibody bound by Biotin-conjugated T4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T4 in the sample. The color development is stopped and the intensity of the color is measured.

96 Tests

625 USD