Human Defensin-5, DEFA5 ELISA Kit | MyBioSource MBS942471 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Defensin-5, DEFA5 ELISA Kit

catalog :

MBS942471

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS942471

products type :

ELISA Kit

products full name :

Human Defensin-5, DEFA5 ELISA Kit

products short name :

defensin, alpha 5, Paneth cell-specific

products name syn :

Human Defensin-5 (DEFA5) ELISA kit; DEF5; HD-5; MGC129728; defensin 5; defensin; alpha 5; defensin; alpha 5; preproprotein; defensin; alpha 5; Paneth cell-specific

other names :

defensin-5 preproprotein; Defensin-5; defensin-5; HD5(20-94); defensin 5; defensin, alpha 5, preproprotein; defensin, alpha 5, Paneth cell-specific; Defensin, alpha 5; HD5(20-94)Cleaved into the following 4 chains:HD5(23-94); HD5(29-94); HD5(56-94); HD5(63-94)

products gene name :

DEFA5

other gene names :

DEFA5; DEFA5; DEF5; HD-5; DEF5

uniprot entry name :

DEF5_HUMAN

reactivity :

Human

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of human DEFA5. No significant cross-reactivity or interference between human DEFA5 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of human DEFA5 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFA5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

10337585

ncbi acc num :

NP_066290.1

ncbi gb acc num :

NM_021010.1

uniprot acc num :

Q01523

ncbi mol weight :

10,071 Da

ncbi pathways :

Alpha-defensins Pathway (530758); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)

ncbi summary :

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum. [provided by RefSeq, Jul 2008]

uniprot summary :

DEFA5: Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing. Belongs to the alpha-defensin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: extracellular space; transport vesicle; Golgi lumen; extracellular region. Biological Process: killing of cells of another organism; antibacterial humoral response; innate immune response; defense response to fungus

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage