catalog number :
MBS9424658
products type :
ELISA Kit
products full name :
Mouse 1, 25-dihydroxyvitamin D3 (DVD/DHVD3) ELISA kit
products short name :
[1, 25-dihydroxyvitamin D3 (DVD/DHVD3)]
products name syn :
[1, 25 DHVD3; 1, 25-dihydroxyvitamin D3]
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse DHVD 3. No significant cross-reactivity or interference between mouse DHVD 3 and analogues was observed.
storage stability :
Store at 4 degree C
other info1 :
Samples: Serum. Detection Range: 1000 fmo1lL-5000 fmoi/L. Sensitivity: <250 fmoilL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<10%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated DHVD 3 Hapten. The competitive inhibition reaction is launched between with HRP-conjugated DHVD 3 Hapten and DHVD 3 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of DHVD 3 in the sample. The color development is stopped and the intensity of the color is measured.