catalog number :
MBS9424421
products type :
ELISA Kit
products full name :
Fish Thyroxine, T4 ELISA Kit
products short name :
[Thyroxine, T4]
products name syn :
[Thyroxine; T4]
products gene name :
[T4]
specificity :
This assay has high sensitivity and excellent specificity for detection of fish T4. No Significant cross-reactivity or interference between fish T4 and analogues was observed.
storage stability :
Store at 4 degree C
other info1 :
Samples: serum, plasma and tissue homogenates. Assay Type: Quantitative Competitive. Detection Range: 20 ng/ml-320ng/ml. Sensitivity: <20 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coaled with an antibody specific to T4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T4. A competitive inhibilion reaction is launched between T4 (Standards or samples) and Biotin-conjugated T4 with the pre-coated antibody specific for T4. The more amount of T4 in samples, the less antibody bound by Biotin-conjugated T 4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T4 in the sample. The color development is stopped and the intensity of the color is measured.