catalog number :
MBS942351
products type :
ELISA Kit
products full name :
Mouse Bone morphogenetic protein 8B, BMP8B ELISA Kit
products short name :
bone morphogenetic protein 8b
products name syn :
Mouse Bone morphogenetic protein 8B (BMP8B) ELISA kit; BMP8; MGC131757; OP2; bone morphogenetic protein 8 (osteogenic protein 2) ; bone morphogenetic protein 8B; dJ118J21.1 (bone morphogenetic protein 8 (osteogenic protein 2) ) ; osteogenic protein 2; bone morphogenetic protein 8b
other names :
bone morphogenetic protein 8B; Bone morphogenetic protein 8B; bone morphogenetic protein 8B; BMP-8B; bone morphogenetic protein 8b
products gene name :
BMP8B
other gene names :
Bmp8b; Bmp8b; Op3; BMP-8B
uniprot entry name :
BMP8B_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse BMP8B. No significant cross-reactivity or interference between mouse BMP8B and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 23.5 pg/ml -1500 pg/ml. Sensitivity: 5.8 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BMP8B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP8B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP8B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP8B bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_031585.2
ncbi gb acc num :
NM_007559.4
ncbi mol weight :
44,943 Da
ncbi pathways :
Hedgehog Signaling Pathway (83260); Hedgehog Signaling Pathway (474); TGF-beta Signaling Pathway (83261); TGF-beta Signaling Pathway (475)
uniprot summary :
BMP8B: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Belongs to the TGF-beta family. Protein type: Secreted, signal peptide; Cytokine; Secreted. Cellular Component: extracellular space; extracellular region. Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding. Biological Process: BMP signaling pathway; regulation of apoptosis; diet induced thermogenesis; ossification; regulation of MAPKKK cascade; cartilage development; multicellular organismal development; transforming growth factor beta receptor signaling pathway; spermatogenesis; embryonic morphogenesis; cell differentiation; growth; germ cell development
size4 :
10x96-Strip-Wells