catalog number :
MBS942323
products type :
ELISA Kit
products full name :
Human Oligodendrocyte-myelin glycoprotein, OMG ELISA Kit
products short name :
oligodendrocyte myelin glycoprotein
products name syn :
Human Oligodendrocyte-myelin glycoprotein (OMG) ELISA kit; OMGP; ; oligodendrocyte myelin glycoprotein
other names :
oligodendrocyte-myelin glycoprotein; Oligodendrocyte-myelin glycoprotein; oligodendrocyte-myelin glycoprotein; oligodendrocyte myelin glycoprotein
other gene names :
OMG; OMG; OMGP; OMGP
uniprot entry name :
OMGP_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human OMG. No significant cross-reactivity or interference between human OMG and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human OMG is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OMG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OMG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OMG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OMG bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_002535.3
ncbi gb acc num :
NM_002544.4
ncbi mol weight :
49,608 Da
ncbi pathways :
Axonal Growth Inhibition (RHOA Activation) Pathway (106454); Signal Transduction Pathway (477114); Signalling By NGF Pathway (106439); P75 NTR Receptor-mediated Signalling Pathway (106441); P75(NTR)-mediated Signaling Pathway (138019); P75NTR Regulates Axonogenesis Pathway (106452)
uniprot summary :
OMG: Cell adhesion molecule contributing to the interactive process required for myelination in the central nervous system. Protein type: Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 17q11.2. Cellular Component: plasma membrane. Molecular Function: identical protein binding. Biological Process: nerve growth factor receptor signaling pathway; neurite regeneration; negative regulation of axonogenesis; regulation of axonogenesis; cell adhesion; regulation of collateral sprouting of intact axon in response to injury
size4 :
10x96-Strip-Wells