catalog number :
MBS9422604
products type :
Recombinant Protein
products full name :
Recombinant Rotavirus A Intermediate capsid protein VP6
products short name :
[Rotavirus A Intermediate capsid protein VP6]
other names :
[Intermediate capsid protein VP6; Intermediate capsid protein VP6]
products gene name :
[VP6]
sequence positions :
[Full Length, 1-397aa]
sequence :
MDVLYSLSKTLKDARDKIVEGTLYSNVSDLIQQFNQMII
TMNGNEFQTGGIGNLPIRNWNFDFGLLGTTLLNLDANYV
ETARNTIDYFVDFVDNVCMDEMVRESQRNGIAPQSDSLI
KLSGIKFKRINFDNSSEYIENWNLQNRRQRTGFTFHKPN
IFPYSASFTLNRSQPAHDNLMGTMWLNAGSEIQVAGFDY
SCAINAPANTQQFEHIVQLRRVLTTATITLLPDAERFSF
PRVITSADGATTWYFNPVILRPNNVEIEFLLNGQIINTY
QARFGTIIARNFDTIRLSFQLMRPPNMTPAVAALFPNAQ
PFEHHATVGLTLRIESAVCESVLADASETMLANVTSVRQ
EYAIPVGPVFPPGMNWTDLITNYSPSREDNLQRVFTVAS
IRSMLVK
purity :
Greater than 90% as determined by SDS-PAGE.
form :
Tris-based buffer50% glycerol
storage stability :
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C,-80°C. The shelf life of lyophilized form is 12 months at -20°C,-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for
up to one week.
other info2 :
Calculated MW: 46.9 kDa. Tag Info: N-terminal 6xHis-tagged. immunogen Description: Expression Region:1-397aaSequence Info:Full Length
products description :
Intermediate capsid protein that self assbles to form an icosahedral capsid with a T=13 symmetry, which consists of 230 trimers of VP6, with channels at each of its five-fold vertices. This capsid constitutes the middle concentric layer of the viral mature particle. The innermost VP2 capsid and the intermediate VP6 capsid rain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP6 is required for the transcription activity of the DLP .
uniprot summary :
Intermediate capsid protein that self assembles to form an icosahedral capsid with a T=13 symmetry, which consists of 230 trimers of VP6, with channels at each of its five-fold vertices. This capsid constitutes the middle concentric layer of the viral mature particle. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nascent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP6 is required for the transcription activity of the DLP ().