Human DNA topoisomerase 2-beta, TOP2B ELISA Kit | MyBioSource MBS942146 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human DNA topoisomerase 2-beta, TOP2B ELISA Kit

catalog :

MBS942146

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS942146

products type :

ELISA Kit

products full name :

Human DNA topoisomerase 2-beta, TOP2B ELISA Kit

products short name :

topoisomerase (DNA) II beta 180kDa

products name syn :

Human DNA topoisomerase 2-beta (TOP2B) ELISA kit; TOPIIB; top2beta; DNA topoisomerase II beta; DNA topoisomerase II; 180 kD; DNA topoisomerase II; beta isozyme; U937 associated antigen; antigen MLAA-44; topo II beta; topoisomerase II beta; topoisomerase I; topoisomerase (DNA) II beta 180kDa

other names :

DNA topoisomerase 2-beta; DNA topoisomerase 2-beta; DNA topoisomerase 2-beta; topo II beta; antigen MLAA-44; topoisomerase IIb; topoisomerase II beta; U937 associated antigen; DNA topoisomerase II beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme; topoisomerase (DNA) II beta 180kDa; DNA topoisomerase II, beta isozyme

products gene name :

TOP2B

other gene names :

TOP2B; TOP2B; TOPIIB; top2beta

uniprot entry name :

TOP2B_HUMAN

reactivity :

Human

sequence length :

1621

specificity :

This assay has high sensitivity and excellent specificity for detection of human TOP2B. No significant cross-reactivity or interference between human TOP2B and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml -1200 pg/ml. Sensitivity: 4.69 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TOP2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TOP2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOP2B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOP2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

19913408

ncbi acc num :

NP_001059.2

ncbi gb acc num :

NM_001068.3

uniprot acc num :

Q02880

ncbi mol weight :

183,267 Da

ncbi summary :

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants; however, the second variant has not yet been fully described. [provided by RefSeq, Jul 2008]

uniprot summary :

TOP2B: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly involved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. Belongs to the type II topoisomerase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell development/differentiation; DNA-binding; Nucleolus; Topoisomerase; EC 5.99.1.3; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 3p24. Cellular Component: nucleoplasm; heterochromatin; DNA topoisomerase complex (ATP-hydrolyzing); cytoplasm; nucleolus; cytosol; nucleus. Molecular Function: protein C-terminus binding; DNA topoisomerase (ATP-hydrolyzing) activity; enzyme binding; protein kinase C binding; protein heterodimerization activity; metal ion binding; histone deacetylase binding; chromatin binding; DNA bending activity; ATP binding. Biological Process: DNA unwinding during replication; mitotic recombination; DNA topological change; axonogenesis; forebrain development; neuron migration; sister chromatid segregation; resolution of meiotic joint molecules as recombinants

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage