product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Centromere protein K, CENPK ELISA Kit
catalog :
MBS942080
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS942080
products type :
ELISA Kit
products full name :
Human Centromere protein K, CENPK ELISA Kit
products short name :
centromere protein K
products name syn :
Human Centromere protein K (CENPK) ELISA kit; AF5alpha; CENP-K; FKSG14; P33; Solt; OTTHUMP00000161888; SoxLZ/Sox6 leucine zipper binding protein; SoxLZ/Sox6-binding protein Solt; leucine zipper protein FKSG14; centromere protein K
other names :
centromere protein K; Centromere protein K; centromere protein K; protein AF-5alpha; leucine zipper protein FKSG14; SoxLZ/Sox6-binding protein Solt; interphase centromere complex protein 37; centromere protein K; Interphase centromere complex protein 37; Protein AF-5alpha; p33
products gene name :
CENPK
other gene names :
CENPK; CENPK; P33; Solt; CENP-K; FKSG14; AF5alpha; ICEN37; FKSG14; CENP-K
uniprot entry name :
CENPK_HUMAN
reactivity :
Human
sequence length :
269
specificity :
This assay has high sensitivity and excellent specificity for detection of Human CENPK. No significant cross-reactivity or interference between Human CENPK and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.5 pg/ml-1500 pg/ml. Sensitivity: 5.8 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CENPK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CENPK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CENPK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CENPK bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
388490144
ncbi acc num :
NP_001253967.1
ncbi gb acc num :
NM_001267038.1
uniprot acc num :
Q9BS16
ncbi mol weight :
31,655 Da
ncbi pathways :
Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Chromosome Maintenance Pathway (161048); DNA Replication Pathway (105897); Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway (161050); M Phase Pathway (105812); Mitotic M-M/G1 Phases Pathway (160943); Mitotic Prometaphase Pathway (105815); Nucleosome Assembly Pathway (161049)
ncbi summary :
CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM, Mar 2008]
uniprot summary :
CENPK: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore. Chromosomal aberrations involving CENPK are a cause of acute leukemias. Translocation t(5;11)(q12;q23) with MLL. Belongs to the CENPK family. Chromosomal Location of Human Ortholog: 5q12.3. Cellular Component: nucleoplasm; cytosol. Biological Process: nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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