ELISA/assay

Human DNA- (apurinic or apyrimidinic site) lyase, APEX1 ELISA Kit

MBS941991

48-Strip-Wells

510 USD

ELISA Kit

Human DNA- (apurinic or apyrimidinic site) lyase, APEX1 ELISA Kit

APEX nuclease (multifunctional DNA repair enzyme) 1

Human DNA- (apurinic or apyrimidinic site) lyase (APEX1) ELISA kit; APE; APE1; APEN; APEX; APX; HAP1; REF1; AP endonuclease class I; AP lyase; APEX nuclease 1; DNA- (apurinic or apyrimidinic site) lyase; apurinic/apyrimidinic (abasic) endonuclease; apurinic/apyrimidinic e; APEX nuclease (multifunctional DNA repair enzyme) 1

DNA-(apurinic or apyrimidinic site) lyase; DNA-(apurinic or apyrimidinic site) lyase; DNA-(apurinic or apyrimidinic site) lyase; DNA-(apurinic or apyrimidinic site) lyase; AP lyase; protein REF-1; redox factor-1; AP endonuclease class I; apurinic-apyrimidinic endonuclease 1; apurinic/apyrimidinic (abasic) endonuclease; deoxyribonuclease (apurinic or apyrimidinic); APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease; APEN; Apurinic-apyrimidinic endonuclease 1; AP endonuclease 1; APE-1; REF-1; Redox factor-1Cleaved into the following chain:DNA-(apurinic or apyrimidinic site) lyase, mitochondrial

APEX1

APEX1; APEX1; APE; APX; APE1; APEN; APEX; HAP1; REF1; APE; APE1; APEX; APX; HAP1; REF1; APEN; AP endonuclease 1; APE-1

APEX1_HUMAN

Human

318

This assay has high sensitivity and excellent specificity for detection of human APEX1. No significant cross-reactivity or interference between human APEX1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: 3.9 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APEX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APEX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APEX1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APEX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

346644849

NP_001231178.1

NM_001244249.1

P27695

35,555 Da

BER Complex Pathway (413429); BER Complex Pathway (468377); Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway (105848); DNA Repair Pathway (105837); Displacement Of DNA Glycosylase By APE1 Pathway (105849); HIF-2-alpha Transcription Factor Network Pathway (137956); Removal Of DNA Patch Containing Abasic Residue Pathway (105851)

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008]

APE1: a multifunctional enzyme that plays a central role in the cellular response to oxidative stress including DNA repair and redox regulation of transcriptional factors. Binds DNA and RNA. Functions as an apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway, a 3 -5 exoribonuclease for mismatched deoxyribonucleotides at the 3 termini of nicked or gapped DNA molecules, and a DNA 3 phosphodiesterase capable of removing lesions (such as phosphoglycolate) blocking the 3 side of DNA strand breaks. Is a loading factor for POLB onto non-incised AP sites in DNA, stimulates the 5 -terminal deoxyribose 5 - phosphate (dRp) excision activity of POLB, and involved in the DNA cleavage step of class switch recombination (CSR). Possesses reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Binds to negative calcium response elements (nCaREs). Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Is an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover. In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Interacts with SIRT1; the interaction is increased in the context of genotoxic stress. Interacts with HDAC1, HDAC2 and HDAC3; the interactions are not dependent on the APEX1 acetylation status. Up-regulated in presence of reactive oxygen species (ROS), like bleomycin, H2O2 and phenazine methosulfate. NPM1 stimulates endodeoxyribonuclease activity on double-stranded DNA with AP sites, but inhibits endoribonuclease activity on single-stranded RNA containing AP sites. Belongs to the DNA repair enzymes AP/ExoA family. Protein type: Deoxyribonuclease; DNA-binding; Endoplasmic reticulum; EC 4.2.99.18; DNA repair, damage; Nucleolus; Hydrolase; Nuclear receptor co-regulator; Lyase; Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: nucleoplasm; centrosome; transcription factor complex; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; nucleolus; ribosome; nuclear speck; nucleus. Molecular Function: phosphodiesterase I activity; uracil DNA N-glycosylase activity; phosphoric diester hydrolase activity; DNA-(apurinic or apyrimidinic site) lyase activity; chromatin DNA binding; metal ion binding; transcription coactivator activity; oxidoreductase activity; endodeoxyribonuclease activity; NF-kappaB binding; protein binding; DNA binding; endonuclease activity; protein complex binding; damaged DNA binding; ribonuclease H activity; 3 -5 exonuclease activity; transcription corepressor activity; double-stranded DNA specific 3 -5 exodeoxyribonuclease activity; site-specific endodeoxyribonuclease activity, specific for altered base. Biological Process: response to drug; positive regulation of DNA repair; mismatch repair; transcription, DNA-dependent; negative regulation of smooth muscle cell migration; DNA strand elongation during DNA replication; DNA repair; regulation of mRNA stability; DNA catabolic process, endonucleolytic; double-strand break repair via homologous recombination; telomere maintenance via semi-conservative replication; regulation of transcription, DNA-dependent; nucleotide-excision repair; cell redox homeostasis; base-excision repair; double-strand break repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA catabolic process, exonucleolytic; telomere maintenance; aging

48-Strip-Wells

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725

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2565

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