Human mannan-binding lectin serine peptidase 2, MASP2 ELISA Kit | MyBioSource MBS941977 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human mannan-binding lectin serine peptidase 2, MASP2 ELISA Kit

catalog :

MBS941977

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS941977

products type :

ELISA Kit

products full name :

Human mannan-binding lectin serine peptidase 2, MASP2 ELISA Kit

products short name :

mannan-binding lectin serine peptidase 2

products name syn :

Human mannan-binding lectin serine peptidase 2 (MASP2) ELISA kit; MAP19; MASP-2; sMAP; MBL-associated plasma protein of 19 kD; MBL-associated serine protease 2; mannan-binding lectin serine protease 2; small MBL-associated protein; mannan-binding lectin serine peptidase 2

other names :

mannan-binding lectin serine protease 2 isoform 1 preproprotein; Mannan-binding lectin serine protease 2; mannan-binding lectin serine protease 2; small MBL-associated protein; MBL-associated serine protease 2; MBL-associated plasma protein of 19 kD; mannan-binding lectin serine protease 1 pseudogene 1; mannose-binding protein-associated serine protease 2; mannan-binding lectin serine peptidase 1 pseudogene 1; mannan-binding lectin serine peptidase 2; MBL-associated serine protease 2; Mannose-binding protein-associated serine protease 2; MASP-2

products gene name :

MASP2

other gene names :

MASP2; MASP2; sMAP; MAP19; MASP-2; MASP1P1; MASP-2

uniprot entry name :

MASP2_HUMAN

reactivity :

Human

sequence length :

686

specificity :

This assay has high sensitivity and excellent specificity for detection of human MASP2. No significant cross-reactivity or interference between human MASP2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates and cerebrospinal fluid (CSF). Assay Type: Sandwich. Detection Range: 23.44 pg/ml -1500 pg/ml. Sensitivity: The minimum detectable dose of human MASP2 is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MASP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MASP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MASP2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MASP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

21264363

ncbi acc num :

NP_006601.2

ncbi gb acc num :

NM_006610.3

uniprot acc num :

O00187

ncbi mol weight :

75,702 Da

ncbi pathways :

Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Creation Of C4 And C2 Activators Pathway (106407); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406); Innate Immune System Pathway (106387); Lectin Pathway Of Complement Activation (106408); Staphylococcus Aureus Infection Pathway (172846)

ncbi summary :

The Ra-reactive factor (RARF) is a complement-dependent bactericidal factor that binds to the Ra and R2 polysaccharides expressed by certain enterobacteria. Alternate splicing of this gene results in two transcript variants encoding two RARF components that are involved in the mannan-binding lectin pathway of complement activation. The longer isoform is cleaved into two chains which form a heterodimer linked by a disulfide bond. The encoded proteins are members of the trypsin family of peptidases. [provided by RefSeq, Jul 2008]

uniprot summary :

MASP2: Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase. Defects in MASP2 are the cause of MASP2 deficiency (MASPD). MASPD is a disorder that results in autoimmune manifestations, recurrent severe infections, and chronic inflammatory disease. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Protease; EC 3.4.21.104; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p36.3-p36.2. Cellular Component: extracellular region. Molecular Function: peptidase activity; protein binding; serine-type endopeptidase activity; complement component C4b binding; calcium ion binding; calcium-dependent protein binding. Biological Process: innate immune response; complement activation, lectin pathway; proteolysis; complement activation, classical pathway; complement activation. Disease: Masp2 Deficiency

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage