catalog number :
MBS941920
products type :
ELISA Kit
products full name :
Mouse Protein FAM132B, FAM132B ELISA Kit
products short name :
family with sequence similarity 132, member B
products name syn :
Mouse Protein FAM132B (FAM132B) ELISA kit; FLJ37034; hypothetical protein LOC151176; family with sequence similarity 132; member B
other names :
protein FAM132B; Erythroferrone; protein FAM132B; complement C1q tumor necrosis factor-related protein 15; family with sequence similarity 132, member B; Complement C1q tumor necrosis factor-related protein 15; Myonectin
products gene name :
FAM132B
other gene names :
Fam132b; Fam132b; myonectin; 4832406C22; C1qtnf15; Ctrp15; Erfe
uniprot entry name :
ERFE_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse FAM132B. No significant cross-reactivity or interference between mouse FAM132B and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml -1200 pg/ml. Sensitivity: The minimum detectable dose of mouse FAM132B is typically less than 4.68 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FAM132B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FAM132B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAM132B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAM132B bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_775571.2
ncbi gb acc num :
NM_173395.2
ncbi mol weight :
36,265 Da
uniprot summary :
FAM132B: Belongs to the FAM132 family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular space; cell; extracellular region. Molecular Function: protein homodimerization activity; hormone activity. Biological Process: cellular iron ion homeostasis; regulation of fatty acid metabolic process
size4 :
10x96-Strip-Wells