Human phosphoglycerate kinase 1, PGK1 ELISA Kit | MyBioSource MBS941880 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human phosphoglycerate kinase 1, PGK1 ELISA Kit

catalog :

MBS941880

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

product information

catalog number :

MBS941880

products type :

ELISA Kit

products full name :

Human phosphoglycerate kinase 1, PGK1 ELISA Kit

products short name :

[phosphoglycerate kinase 1]

products name syn :

[Human phosphoglycerate kinase 1 (PGK1) ELISA kit; RP4-570L12.1; MGC117307; MGC142128; MGC8947; MIG10; PGKA; cell migration-inducing gene 10 protein; primer recognition protein 2; phosphoglycerate kinase 1]

other names :

[phosphoglycerate kinase 1; Phosphoglycerate kinase 1; phosphoglycerate kinase 1; PRP 2; primer recognition protein 2; cell migration-inducing gene 10 protein; epididymis secretory sperm binding protein Li 68p; phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2; PRP 2]

products gene name :

[PGK1]

other gene names :

[PGK1; PGK1; PGKA; MIG10; HEL-S-68p; PGKA; MIG10; OK/SW-cl.110; PRP 2]

uniprot entry name :

PGK1_HUMAN

reactivity :

Human

sequence length :

417

specificity :

This assay has high sensitivity and excellent specificity for detection of human PGK1. No significant cross-reactivity or interference between human PGK1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 28 pg/ml-1800 pg/ml. Sensitivity: The minimum detectable dose of human PGK1 is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PGK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PGK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGK1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4505763

ncbi acc num :

NP_000282.1

ncbi gb acc num :

NM_000291.3

uniprot acc num :

P00558

ncbi mol weight :

44,615 Da

ncbi pathways :

Gluconeogenesis Pathway (106204); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (413342); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196); Glucose Metabolism Pathway (106199); Glycolysis Pathway (105911); Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate (413340); Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate (468194); Glycolysis / Gluconeogenesis Pathway (82926); Glycolysis / Gluconeogenesis Pathway (287); Glycolysis And Gluconeogenesis Pathway (198814)

ncbi summary :

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]

uniprot summary :

PGK1: an apparent multifunctional protein. A glycolytic enzyme and a polymerase alpha cofactor protein (primer recognition protein). Protein type: EC 2.7.2.3; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other. Chromosomal Location of Human Ortholog: Xq13.3. Cellular Component: membrane; cytosol. Molecular Function: protein binding; phosphoglycerate kinase activity; ATP binding. Biological Process: glycolysis; epithelial cell differentiation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; phosphorylation; gluconeogenesis. Disease: Phosphoglycerate Kinase 1 Deficiency

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

550

size3 :

96-Strip-Wells

price3 :

795

size4 :

5x96-Strip-Wells

price4 :

2890

size5 :

10x96-Strip-Wells

price5 :

5465