product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Urocortin, UCN ELISA Kit
catalog :
MBS941797
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS941797
products type :
ELISA Kit
products full name :
Mouse Urocortin, UCN ELISA Kit
products short name :
urocortin
products name syn :
Mouse Urocortin (UCN) ELISA kit; MGC129974; MGC129975; UI; UROC; urocortin; preproprotein; urocortin
other names :
urocortin; Urocortin; urocortin; urocortin
products gene name :
UCN
other gene names :
Ucn; Ucn; Ucn1; Mpv17
uniprot entry name :
UCN1_MOUSE
reactivity :
Mouse
sequence length :
122
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse urocortin. No significant cross-reactivity or interference between mouse urocortin and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 12.5 pg/ml-800 pg/ml. Sensitivity: The minimum detectable dose of mouse urocortin is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for urocortin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any urocortin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for urocortin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of urocortin bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
12025528
ncbi acc num :
NP_067265.1
ncbi gb acc num :
NM_021290.2
uniprot acc num :
P81615
ncbi mol weight :
13,558 Da
uniprot summary :
UCN: Acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH). Binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: cell soma; axon; dendrite; extracellular region; perikaryon; nerve terminal. Molecular Function: corticotropin-releasing hormone receptor 1 binding; histone deacetylase inhibitor activity; G-protein-coupled receptor binding; hormone activity; corticotropin-releasing hormone receptor 2 binding. Biological Process: regulation of synaptic transmission, glutamatergic; positive regulation of translation; negative regulation of hormone secretion; positive regulation of collagen biosynthetic process; drinking behavior; negative regulation of cell size; pancreatic juice secretion; positive regulation of vasodilation; negative regulation of appetite; positive regulation of adrenocorticotropic hormone secretion; sensory perception of sound; learning and/or memory; neuropeptide signaling pathway; positive regulation of cAMP biosynthetic process; feeding behavior; negative regulation of blood pressure; neurite development; associative learning; negative regulation of histone deacetylation; activation of protein kinase A; positive regulation of interleukin-6 production; social behavior; positive regulation of cell growth; positive regulation of peptidyl-serine phosphorylation; positive regulation of vascular permeability; negative regulation of catalytic activity; aerobic respiration; response to oxidative stress; positive regulation of protein amino acid phosphorylation; positive regulation of DNA replication; negative regulation of apoptosis
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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