GLI1 Antibody | MyBioSource MBS9417345 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

GLI1 Antibody

catalog :

MBS9417345

quantity :

0.1 mL

price :

255 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Gel: 6%SDS-PAGE Lysate: 40 ug, Lane 1-4: NIH/3T3£¬Raji£¬PC-3 and Hepg2 cell, Primary antibody:GLI1 antibody at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds

image 2 :

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using GLI1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)

image 3 :

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using GLI1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)

product information

catalog number :

MBS9417345

products type :

Antibody

products full name :

GLI1 Antibody

products short name :

[GLI1]

products name syn :

[GLI]

other names :

[zinc finger protein GLI1 isoform 2; Zinc finger protein GLI1; zinc finger protein GLI1; GLI family zinc finger 1; Glioma-associated oncogene; Oncogene GLI]

products gene name :

[GLI1]

other gene names :

[GLI1; GLI1; GLI; GLI]

uniprot entry name :

GLI1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

978

specificity :

The antibody detects endogenous levels of total GLI1 protein.

purity :

Antigen affinity purification

form :

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

concentration :

0.5mg/ml

storage stability :

Store at -20°C

tested application :

Immunohistochemistry (IHC), Western Blot (WB)

app notes :

Western Blot: 1:200-1000. Immunohistochemistry: 1: 20-100

image1 heading :

SDS-Page

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: Synthetic peptide of human GLI1. Immunogen Type: Peptide

other info2 :

. Calculated MW: 118kd

products categories :

Total protein Ab

products description :

This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.

ncbi gi num :

229892345

ncbi acc num :

NP_001153517.1

ncbi gb acc num :

NM_001160045.1

uniprot acc num :

P08151

ncbi pathways :

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Degradation Of GLI1 By The Proteasome Pathway (1269639); Dopaminergic Neurogenesis Pathway (1458255); GLI Proteins Bind Promoters Of Hh Responsive Genes To Promote Transcription Pathway (1269643); Hedgehog 'off' State Pathway (1269636); Hedgehog 'on' State Pathway (1269640); Hedgehog Signaling Pathway (198835); Hedgehog Signaling Pathway (1084754); Hedgehog Signaling Pathway (1108217)

ncbi summary :

uniprot summary :

GLI1: Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. Interacts with KIF7. Interacts with ZIC1; the interaction enhances transcription activation. Amplified in glioblastoma cells. Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. Belongs to the GLI C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: C2H2-type zinc finger protein; Oncoprotein; Transcription factor. Chromosomal Location of Human Ortholog: 12q13.2-q13.3. Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; nucleoplasm; nucleus. Molecular Function: DNA binding; protein binding. Biological Process: digestive tract morphogenesis; epidermal cell differentiation; osteoblast differentiation; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of smoothened signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of smoothened signaling pathway; smoothened signaling pathway

size1 :

0.1 mL

price1 :

255 USD

more info or order :

MyBioSource product webpage