Mouse Calcium-dependent phospholipase A2, PLA2G5 ELISA Kit | MyBioSource MBS941705 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Calcium-dependent phospholipase A2, PLA2G5 ELISA Kit

catalog :

MBS941705

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS941705

products type :

ELISA Kit

products full name :

Mouse Calcium-dependent phospholipase A2, PLA2G5 ELISA Kit

products short name :

phospholipase A2, group V

products name syn :

Mouse Calcium-dependent phospholipase A2 (PLA2G5) ELISA kit; DKFZp686C2294; GV-PLA2; MGC46205; PLA2-10; hVPLA (2); Ca2+-dependent phospholipase A2; phosphatidylcholine 2-acylhydrolase; phospholipase A2; group V

other names :

calcium-dependent phospholipase A2; Calcium-dependent phospholipase A2; calcium-dependent phospholipase A2; PLA2-10; group V phospholipase A2; phosphatidylcholine 2-acylhydrolase 5; phospholipase A2, group V; Group V phospholipase A2; PLA2-10; Phosphatidylcholine 2-acylhydrolase 5

products gene name :

PLA2G5

other gene names :

Pla2g5; Pla2g5; PLA2; sPLA2

uniprot entry name :

PA2G5_MOUSE

reactivity :

Mouse

sequence length :

137

specificity :

This assay has high sensitivity and excellent specificity for detection of Mouse PLA2G5. No significant cross-reactivity or interference between Mouse PLA2G5 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Lipogenesis and lipometabolism. Protein Biological Process 3: Lipid degradation

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLA2G5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLA2G5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

171906563

ncbi acc num :

NP_001116426.1

ncbi gb acc num :

NM_001122954.1

uniprot acc num :

P97391

ncbi mol weight :

15,859 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (83190); Arachidonic Acid Metabolism Pathway (366); Arachidonic Acid Metabolism Pathway (522975); Ether Lipid Metabolism Pathway (83189); Ether Lipid Metabolism Pathway (365); Fat Digestion And Absorption Pathway (194386); Fat Digestion And Absorption Pathway (194324); Fc Epsilon RI Signaling Pathway (83279); Fc Epsilon RI Signaling Pathway (493); Glutamatergic Synapse Pathway (213819)

uniprot summary :

PLA2G5: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L- alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L- alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle. Defects in PLA2G5 are the cause of fleck retina, familial benign (FRFB). An autosomal recessive condition associated with a distinctive retinal appearance and no apparent visual or electrophysiologic deficits. Affected individuals are asymptomatic, but fundus examination reveals a striking pattern of diffuse, yellow-white, fleck-like lesions extending to the far periphery of the retina but sparing the foveal region. Belongs to the phospholipase A2 family. Protein type: Phospholipase; Cell surface; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - arachidonic acid; Secreted; Lipid Metabolism - alpha-linolenic acid; EC 3.1.1.4; Secreted, signal peptide; Lipid Metabolism - ether lipid; Lipid Metabolism - linoleic acid. Cellular Component: Golgi apparatus; cell surface; membrane; perinuclear region of cytoplasm; extracellular region; plasma membrane. Molecular Function: heparin binding; phospholipase A2 activity; hydrolase activity; calcium-dependent phospholipase A2 activity; metal ion binding; calcium ion binding; receptor binding. Biological Process: leukotriene biosynthetic process; platelet activating factor biosynthetic process; phospholipid metabolic process; lipid metabolic process; arachidonic acid secretion; lipid catabolic process

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage