Human Protein Wnt-5b, WNT5B ELISA Kit | MyBioSource MBS941658 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Protein Wnt-5b, WNT5B ELISA Kit

catalog :

MBS941658

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS941658

products type :

ELISA Kit

products full name :

Human Protein Wnt-5b, WNT5B ELISA Kit

products short name :

wingless-type MMTV integration site family, member 5B

products name syn :

Human Protein Wnt-5b (WNT5B) ELISA kit; MGC2648; WNT-5B protein; wingless-type MMTV integration site family; member 5B

other names :

protein Wnt-5b; Protein Wnt-5b; protein Wnt-5b; WNT-5B protein; wingless-type MMTV integration site family, member 5B

products gene name :

WNT5B

other gene names :

WNT5B; WNT5B

uniprot entry name :

WNT5B_HUMAN

reactivity :

Human

sequence length :

359

specificity :

This assay has high sensitivity and excellent specificity for detection of human WNT5B. No significant cross-reactivity or interference between human WNT5B and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human WNT5B is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT5B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT5B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT5B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT5B bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

17402919

ncbi acc num :

NP_110402.2

ncbi gb acc num :

NM_030775.2

uniprot acc num :

Q9H1J7

ncbi mol weight :

40,323 Da

ncbi pathways :

Adipogenesis Pathway (198832); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); DNA Damage Response (only ATM Dependent) Pathway (198827); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83063); Hedgehog Signaling Pathway (474); Melanogenesis Pathway (83092); Melanogenesis Pathway (504)

ncbi summary :

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]

uniprot summary :

WNT5B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Interacts with PORCN. Belongs to the Wnt family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12p13.3. Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; Golgi lumen; endoplasmic reticulum lumen; plasma membrane; extracellular region. Molecular Function: frizzled binding; receptor binding. Biological Process: fat cell differentiation; neuron differentiation; cell fate commitment; Wnt receptor signaling pathway; wound healing; chondrocyte differentiation; positive regulation of fat cell differentiation; positive regulation of cell migration

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage