catalog number :
MBS941614
products type :
ELISA Kit
products full name :
Mouse Protein-glutamine gamma-glutamyltransferase K, TGM1 ELISA Kit
products short name :
transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase)
products name syn :
Mouse Protein-glutamine gamma-glutamyltransferase K (TGM1) ELISA kit; ICR2; KTG; LI; LI1; TGASE; TGK; TGase K; epidermal TGase; transglutaminase 1; transglutaminase K; transglutaminase; keratinocyte; transglutaminase 1 (K polypeptide epidermal type I; protein-glutamine-gamma-glutamyltransferase)
other names :
protein-glutamine gamma-glutamyltransferase K; Protein-glutamine gamma-glutamyltransferase K; protein-glutamine gamma-glutamyltransferase K; TGK; TG K; TGase 1; TGase K; TGase-1; K polypeptide; epidermal TGase; transglutaminase K; transglutaminase-1; transglutaminase type 1; protein-glutamine-gamma-glutamyltransferase; transglutaminase 1, K polypeptide; Epidermal TGase; Transglutaminase K; TG(K); TGK; TGase K; Transglutaminase-1; TGase-1
products gene name :
TGM1
other gene names :
Tgm1; Tgm1; Tgase1; 2310004J08Rik; TG(K); TGK; TGase K; TGase-1
uniprot entry name :
TGM1_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse TGM1. No significant cross-reactivity or interference between Mouse TGM1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 2.35 mU/ml-150 mU/ml. Sensitivity: 0.58 mU/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Keratinization
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TGM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGM1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001155186.1
ncbi gb acc num :
NM_001161714.1
ncbi mol weight :
89,826 Da
uniprot summary :
TGM1: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. Responsible for cross-linking epidermal proteins during formation of the stratum corneum. Protein type: EC 2.3.2.13; Transferase. Cellular Component: cell-cell adherens junction; membrane; intrinsic to membrane. Molecular Function: transferase activity; protein-glutamine gamma-glutamyltransferase activity; metal ion binding; transferase activity, transferring acyl groups. Biological Process: keratinocyte differentiation; organ morphogenesis; keratinization; protein metabolic process; peptide cross-linking
size4 :
10x96-Strip-Wells