catalog number :
MBS941264
products type :
ELISA Kit
products full name :
Mouse Semaphorin-3D, SEMA3D ELISA Kit
products short name :
sema domain, immunoglobulin domain (Ig) , short basic domain, secreted, (semaphorin) 3D
products name syn :
Mouse Semaphorin-3D (SEMA3D) ELISA kit; MGC39708; Sema-Z2; coll-2; collapsin 2; semaphorin 3D; sema domain; immunoglobulin domain (Ig); short basic domain; secreted; (semaphorin) 3D
other names :
semaphorin-3D; Semaphorin-3D; semaphorin-3D; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D
products gene name :
SEMA3D
other gene names :
Sema3d; Sema3d; 4631426B19Rik
uniprot entry name :
SEM3D_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse SEMA3D. No significant cross-reactivity or interference between Mouse SEMA3D and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 23.5 pg/ml-1500 pg/ml. Sensitivity: 5.8 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Differentiation
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEMA3D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA3D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3D is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3D bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_083158.3
ncbi gb acc num :
NM_028882.4
ncbi mol weight :
89,549 Da
ncbi pathways :
Axon Guidance Pathway (83262); Axon Guidance Pathway (476)
uniprot summary :
SEMA3D: a secreted protein that induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin. Protein type: Secreted; Secreted, signal peptide. Cellular Component: membrane; extracellular region. Molecular Function: receptor activity. Biological Process: nervous system development; multicellular organismal development; cell differentiation
size4 :
10x96-Strip-Wells