DAX-1 antibody | MyBioSource MBS9412522 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

DAX-1 antibody

catalog :

MBS9412522

quantity :

0.1 mL

price :

320 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Sample (30 ug of whole cell lysate)A: HeLa S3 7.5% SDS PAGEPrimary antibody diluted at 1: 500

image 2 :

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NR0B1 antibody at 1: 100 dilution.

image 3 :

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DAX-1 antibody at 1: 200 dilution.

product information

catalog number :

MBS9412522

products type :

Antibody

products full name :

DAX-1 antibody

products short name :

[DAX-1]

other names :

[nuclear receptor subfamily 0 group B member 1; Nuclear receptor subfamily 0 group B member 1; nuclear receptor subfamily 0 group B member 1; nuclear receptor subfamily 0, group B, member 1; DSS-AHC critical region on the X chromosome protein 1; Nuclear receptor DAX-1]

products gene name :

[DAX-1]

other gene names :

[NR0B1; NR0B1; AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2; AHC; DAX1]

uniprot entry name :

NR0B1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human

sequence length :

470

purity :

Purified by antigen-affinity chromatography.

form :

Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

concentration :

0.4mg/ml

storage stability :

Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

Western blotting: 1:500-1:3000. Immunohistochemistry: 1:100-1:250. Immunofluorescence: 1:100-1:200

image1 heading :

Testing Data

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen Type: Recombinant protein. Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 96 and 376 of DAX-1

other info2 :

Target Name: DAX-1

products categories :

Total protein Ab

products description :

This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. [provided by RefSeq]

ncbi gi num :

5016090

ncbi acc num :

NP_000466

ncbi gb acc num :

NM_000475.4

ncbi mol weight :

Predicted: 52kd

ncbi pathways :

Gene Expression Pathway (1269649); Generic Transcription Pathway (1269650); Nuclear Receptor Transcription Pathway (1269652); Nuclear Receptors Pathway (198848); Regulation Of Androgen Receptor Activity Pathway (138027)

ncbi summary :

uniprot summary :

NR0B1: orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency. Homodimerizes with STF-1, NR5A2, NR0B2 and with COPS2. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus. Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with STF-1 and NROB2 through its N-terminal LXXLL motifs. Defects in NR0B1 are the cause of congenital X-linked adrenal hypoplasia. Two alternatively spliced human isoforms have been described. Protein type: Nuclear receptor. Chromosomal Location of Human Ortholog: Xp21.3. Cellular Component: nucleoplasm; polysomal ribosome; membrane; cytoplasm; nucleus. Molecular Function: protein domain specific binding; ligand-dependent nuclear receptor activity; protein homodimerization activity; RNA binding; transcription factor binding; steroid hormone receptor binding; DNA hairpin binding; protein binding; DNA binding; AF-2 domain binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription corepressor activity. Biological Process: transcription initiation from RNA polymerase II promoter; hypothalamus development; negative regulation of steroid hormone receptor signaling pathway; gonad development; intracellular receptor-mediated signaling pathway; adrenal gland development; negative regulation of transcription factor activity; male gonad development; Sertoli cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell differentiation; protein localization; Leydig cell differentiation; pituitary gland development; male sex determination; steroid hormone mediated signaling; gene expression; spermatogenesis; negative regulation of transcription, DNA-dependent; steroid biosynthetic process. Disease: 46,xy Sex Reversal 2; Adrenal Hypoplasia, Congenital

size1 :

0.1 mL

price1 :

320 USD

more info or order :

MyBioSource product webpage