Chk2 (Phospho-Thr68) Polyclonal Antibody | MyBioSource MBS9412360 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Chk2 (Phospho-Thr68) Polyclonal Antibody

catalog :

MBS9412360

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot analysis of HELA cells using Phospho-Chk2 (T68) Polyclonal Antibody

image 2 :

Western Blot analysis of Jurkat-UV cells using Phospho-Chk2 (T68) Polyclonal Antibody

product information

catalog number :

MBS9412360

products type :

Antibody

products full name :

Chk2 (Phospho-Thr68) Polyclonal Antibody

products short name :

[Chk2]

products name syn :

[CHEK2; CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2]

other names :

[serine/threonine-protein kinase Chk2 isoform c; Serine/threonine-protein kinase Chk2; serine/threonine-protein kinase Chk2; checkpoint kinase 2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2]

products gene name :

[Chk2]

other gene names :

[CHEK2; CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425; CDS1; CHK2; RAD53; Hucds1; hCds1]

uniprot entry name :

CHK2_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

586

specificity :

Phospho-Chk2 (T68) Polyclonal Antibody detects endogenous levels of Chk2 protein only when phosphorylated at T68.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

form :

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

concentration :

1 mg/ml

storage stability :

Store at -20°C/1 year

tested application :

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

app notes :

Western blotting: 1:500 - 1:2000. Immunohistochemistry: 1:100 - 1:300. ELISA: 1:20000. Not yet tested in other applications

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen Type: peptide. Immunogen Description: Synthesized peptide derived from human Chk2 around the phosphorylation site of T68.

other info2 :

Modification: Phospho. Target Species: Human. Target Name: Chk2

products categories :

Phospho-specific Ab

ncbi gi num :

54112407

ncbi acc num :

NP_001005735.1

ncbi gb acc num :

NM_001005735.1

uniprot acc num :

O96017

ncbi mol weight :

61kd

ncbi pathways :

Cell Cycle Pathway (1269741); Cell Cycle Checkpoints Pathway (1269742); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); DNA Damage Response Pathway (198788); DNA Damage-induced Cell Cycle Checkpoints Pathway (1084767); DNA Damage-induced Cell Cycle Checkpoints Pathway (1108230); FOXM1 Transcription Factor Network Pathway (137935); G1/S DNA Damage Checkpoints Pathway (1269743)

ncbi summary :

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

uniprot summary :

Chk2: a CAMK protein kinase of the Rad53 family. A cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. Phosphorylates and inhibits CDC25C, preventing entry into mitosis, p53, leading to cell cycle arrest in G1, and BRCA1, restoring survival after DNA damage. Twelve splice variant isoforms have been described for human Chk2. LOF mutants cause Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype also caused by p53 mutations. Familial mutations also associated with prostate and breast cancer, and mutations also seen in a variety of sporadic cancers and cell lines. Protein type: EC 2.7.11.1; Protein kinase, CAMK; Tumor suppressor; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; RAD53 family. Chromosomal Location of Human Ortholog: 22q12.1. Cellular Component: nucleoplasm; Golgi apparatus; PML body; chromosome, telomeric region. Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; protein homodimerization activity; metal ion binding; ubiquitin protein ligase binding; protein kinase binding; ATP binding. Biological Process: protein stabilization; transcription, DNA-dependent; DNA damage induced protein phosphorylation; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; DNA damage response, signal transduction; protein amino acid phosphorylation; DNA damage response, signal transduction resulting in induction of apoptosis; regulation of transcription, DNA-dependent; cellular protein catabolic process; cell division; double-strand break repair; DNA damage checkpoint; response to gamma radiation; regulation of protein catabolic process; G2/M transition of mitotic cell cycle; response to DNA damage stimulus. Disease: Li-fraumeni Syndrome 2; Prostate Cancer; Breast Cancer; Osteogenic Sarcoma

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

255

more info or order :

MyBioSource product webpage