SOCS3 Antibody | MyBioSource MBS9411352 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

SOCS3 Antibody

catalog :

MBS9411352

quantity :

0.1 mL

price :

255 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Gel: 8%SDS-PAGE. Lysates (from left to right): Mouse heart tissue . Amount of lysate: 40ug per lane. Primary antibody: 1/300 dilution. Secondary antibody dilution: 1/8000. Exposure time: 20 seconds

image 2 :

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/20.

product information

catalog number :

MBS9411352

products type :

Antibody

products full name :

SOCS3 Antibody

products short name :

[SOCS3]

products name syn :

[CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3]

other names :

[suppressor of cytokine signaling 3; Suppressor of cytokine signaling 3; suppressor of cytokine signaling 3; suppressor of cytokine signaling 3; Cytokine-inducible SH2 protein 3; CIS-3; STAT-induced STAT inhibitor 3; SSI-3]

products gene name :

[SOCS3]

other gene names :

[SOCS3; SOCS3; CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3; CIS3; SSI3; SOCS-3; CIS-3; SSI-3]

uniprot entry name :

SOCS3_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

225

specificity :

The antibody detects endogenous levels of total SOCS3 protein.

purity :

Antigen affinity purification.

form :

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

concentration :

0.6 mg/ml

storage stability :

Store at -20°C

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Western blotting: 1:500-1:2000. Immunohistochemistry: 1:25-1:100

image1 heading :

Testing Data

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen Type: Recombinant Protein. Immunogen Description: Full length fusion protein

other info2 :

Target Name: SOCS3 . SDS-PAGE MW: 25 kD

products categories :

Total protein Ab

products description :

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.

ncbi gi num :

45439352

ncbi acc num :

NP_003946.3

ncbi gb acc num :

NM_003955.4

uniprot acc num :

O14543

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Adaptive Immune System Pathway (1269171); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (1269193); Class I MHC Mediated Antigen Processing Presentation Pathway (1269192); Cytokine Signaling In Immune System Pathway (1269310); ECS Complex Pathway (413455); ECS Complex Pathway (468394)

ncbi summary :

uniprot summary :

SOCS3: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo. Probable substrate recognition component of a SCF-like ECS (Elongin BC- CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST. Interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. Binding to JAK2 is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK2 JH1 domain. Binds specific activated tyrosine residues of the leptin, EPO, IL12, GSCF and gp130 receptors. Interaction with CSNK1E stabilizes SOCS3 protein. Component of the probable ECS(SOCS3) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SOCS3. Interacts with CUL5, RNF7, TCEB1 and TCEB2. Interacts with CUL2. Interacts with FGFR3. Interacts with INSR. Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus. Protein type: Inhibitor. Chromosomal Location of Human Ortholog: 17q25.3. Cellular Component: cytoplasm; cytosol. Molecular Function: protein binding; protein kinase inhibitor activity. Biological Process: regulation of growth; negative regulation of inflammatory response; cytokine and chemokine mediated signaling pathway; protein ubiquitination; negative regulation of protein kinase activity; negative regulation of insulin receptor signaling pathway; JAK-STAT cascade; positive regulation of cell differentiation; negative regulation of JAK-STAT cascade; negative regulation of apoptosis. Disease: Dermatitis, Atopic, 4

size1 :

0.1 mL

price1 :

255 USD

more info or order :

MyBioSource product webpage