WT1 Antibody | MyBioSource MBS9410783 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

WT1 Antibody

catalog :

MBS9410783

quantity :

0.1 mL

price :

255 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of kidney cell lysate using WT1 antibody.

image 2 :

Immunohistochemical analysis of paraffin-embedded human kidney cancer using WT1 antibody at dilution of 1:500 (400x lens).

image 3 :

Immunohistochemical analysis of paraffin-embedded rat lung using WT1 antibody at dilution of 1:500 (400x lens).

product information

catalog number :

MBS9410783

products type :

Antibody

products full name :

WT1 Antibody

products short name :

[WT1]

products name syn :

[GUD; AWT1; WAGR; WT33; NPHS4]

other names :

[Wilms tumor protein isoform A; Wilms tumor protein; Wilms tumor protein; Wilms tumor 1; WT33]

products gene name :

[WT1]

other gene names :

[WT1; WT1; GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1]

uniprot entry name :

WT1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

497

specificity :

The antibody detects endogenous level of total WT1 protein.

purity :

Antibodies were purified by affinity purification using immunogen.

form :

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1.0 mg/ml

storage stability :

Store at -20°C

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

Western blotting: 1:500 - 1:2000. Immunohistochemistry: 1:50 - 1:100. Immunofluorescence: 1:50 - 1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunofluorescence (IF)

image4 description :

Immunofluorescence analysis of HeLa cell using WT1 antibody. Blue: DAPI for nuclear staining.

other info1 :

Immunogen Type: Recombinant Protein. Immunogen Description: Recombinant protein of human WT1. SDS-PAGE MW: 49kD

other info2 :

Target Name: WT1

products categories :

Total protein Ab

products description :

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

ncbi gi num :

65507714

ncbi acc num :

NP_000369.3

ncbi gb acc num :

NM_000378.4

uniprot acc num :

P19544

ncbi pathways :

Integrated Pancreatic Cancer Pathway (711360); Regulation Of Telomerase Pathway (137987); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

ncbi summary :

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]

uniprot summary :

Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).

size1 :

0.1 mL

price1 :

255 USD

more info or order :

MyBioSource product webpage