product summary
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company name :
MyBioSource
product type :
antibody
product name :
U2AF2 Antibody
catalog :
MBS9410392
quantity :
0.1 mL
price :
255 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
image
image 1 :
Western blot analysis of extracts of various cell lines, using U2AF2 antibody.
image 2 :
Immunofluorescence analysis of U2OS cell using U2AF2 antibody. Blue: DAPI for nuclear staining.
product information
catalog number :
MBS9410392
products type :
Antibody
products full name :
U2AF2 Antibody
products short name :
[U2AF2]
products name syn :
[U2AF65]
other names :
[splicing factor U2AF 65 kDa subunit isoform b; Splicing factor U2AF 65 kDa subunit; splicing factor U2AF 65 kDa subunit; U2 small nuclear RNA auxiliary factor 2; U2 auxiliary factor 65 kDa subunit; hU2AF(65); hU2AF65; U2 snRNP auxiliary factor large subunit]
products gene name :
[U2AF2]
other gene names :
[U2AF2; U2AF2; U2AF65; U2AF65; hU2AF(65); hU2AF65]
uniprot entry name :
U2AF2_HUMAN
clonality :
Polyclonal
host :
Rabbit
reactivity :
Human, Mouse, Rat
sequence length :
471
specificity :
The antibody detects endogenous level of total U2AF2 protein.
purity :
Antibodies were purified by affinity purification using immunogen.
form :
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
concentration :
1.0 mg/ml
storage stability :
Store at -20 degree C
tested application :
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
app notes :
Western blotting: 1:500 - 1:2000. Immunohistochemistry: 1:50 - 1:100. Immunofluorescence: 1:50 - 1:200
image1 heading :
Western Blot (WB)
image2 heading :
Immunofluorescence (IF)
other info1 :
Immunogen Type: Recombinant Protein. Immunogen Description: Recombinant protein of human U2AF2.
other info2 :
Target Name: U2AF2
products categories :
Total protein Ab
products description :
U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.
ncbi gi num :
60279268
ncbi acc num :
NP_001012496.1
ncbi gb acc num :
NM_001012478.1
uniprot acc num :
P26368
ncbi mol weight :
53KD
ncbi pathways :
Cleavage Of Growing Transcript In The Termination Region Pathway (1269686); Gene Expression Pathway (1269649); Processing Of Capped Intron-Containing Pre-mRNA Pathway (1269688); RNA Polymerase II Transcription Pathway (1269675); RNA Polymerase II Transcription Termination Pathway (1269685); Spliceosome Pathway (125136); Spliceosome Pathway (124832); Transport Of Mature Transcript To Cytoplasm Pathway (1269693); Transport Of Mature MRNA Derived From An Intron-Containing Transcript Pathway (1269694); MRNA 3'-end Processing Pathway (1269692)
ncbi summary :
U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq, Jul 2008]
uniprot summary :
U2AF2: Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Belongs to the splicing factor SR family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: RNA-binding; Spliceosome; RNA splicing. Chromosomal Location of Human Ortholog: 19q13.42. Cellular Component: nucleoplasm; spliceosome; nuclear speck. Molecular Function: protein binding; enzyme binding; nucleotide binding. Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA 3 -end processing; mRNA processing; termination of RNA polymerase II transcription; positive regulation of RNA splicing
size1 :
0.1 mL
price1 :
255 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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