AP2S1 Antibody | MyBioSource MBS9410212 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

AP2S1 Antibody

catalog :

MBS9410212

quantity :

0.1 mL

price :

255 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Gel: 15+12%SDS-PAGE Lysates (from left to right): Human fetal muscle tissue Amount of lysate: 40ug per lane Primary antibody: 1/250 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute

product information

catalog number :

MBS9410212

products type :

Antibody

products full name :

AP2S1 Antibody

products short name :

[AP2S1]

products name syn :

[AP17; HHC3; CLAPS2; AP17-DELTA]

other names :

[AP-2 complex subunit sigma isoform 3; AP-2 complex subunit sigma; AP-2 complex subunit sigma; adaptor-related protein complex 2, sigma 1 subunit; Adaptor protein complex AP-2 subunit sigma; Adaptor-related protein complex 2 subunit sigma; Clathrin assembly protein 2 sigma small chain; Clathrin coat assembly protein AP17; Clathrin coat-associated protein AP17; HA2 17 kDa subunit; Plasma membrane adaptor AP-2 17 kDa protein; Sigma2-adaptin]

products gene name :

[AP2S1]

other gene names :

[AP2S1; AP2S1; AP17; FBH3; HHC3; FBHOk; CLAPS2; AP17; CLAPS2]

uniprot entry name :

AP2S1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

158

specificity :

The antibody detects endogenous levels of total AP2S1 protein.

purity :

Antigen affinity purification.

form :

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

concentration :

0.9 mg/ml

storage stability :

Store at -20 degree C

tested application :

Western Blot (WB)

app notes :

Western blotting: 1:200-1:1000

image1 heading :

Testing Data

other info1 :

Immunogen Type: Recombinant Protein. Immunogen Description: Full length fusion protein

other info2 :

Target Name: AP2S1

products categories :

Total protein Ab

products description :

One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing has been observed in this gene and results in two known transcripts.

ncbi gi num :

666637993

ncbi acc num :

NP_001288005.1

ncbi gb acc num :

NM_001301076.1

uniprot acc num :

BC006337

ncbi mol weight :

17kd

ncbi pathways :

Adaptive Immune System Pathway (1269171); Axon Guidance Pathway (1270303); Developmental Biology Pathway (1270302); Disease Pathway (1268854); EGFR Downregulation Pathway (1269385); EPH-Ephrin Signaling Pathway (1270330); EPH-ephrin Mediated Repulsion Of Cells Pathway (1270334); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213307); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213276); Endocytosis Pathway (102279)

ncbi summary :

One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

uniprot summary :

AP2S1: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. Belongs to the adaptor complexes small subunit family. 2 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 19q13.32. Cellular Component: plasma membrane; AP-2 adaptor complex; cytosol. Molecular Function: transporter activity; protein transporter activity. Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; synaptic transmission; intracellular protein transport; axon guidance; clathrin cage assembly; viral reproduction; nerve growth factor receptor signaling pathway; regulation of endocytosis; ephrin receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of defense response to virus by virus. Disease: Hypocalciuric Hypercalcemia, Familial, Type Iii

size1 :

0.1 mL

price1 :

255 USD

more info or order :

MyBioSource product webpage