TUT1 Antibody | MyBioSource MBS9409644 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

TUT1 Antibody

catalog :

MBS9409644

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts from 3T3 cells, using TUT1 antiobdy.

image 2 :

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using TUT1 antibody.

product information

catalog number :

MBS9409644

products type :

Antibody

products full name :

TUT1 Antibody

products short name :

[TUT1]

products name syn :

[U6 snRNA-specific terminal uridylyltransferase 1; EC 2.7.7.52; U6-TUTase; RNA-binding protein 21; RNA-binding motif protein 21]

other names :

[speckle targeted PIP5K1A-regulated poly(A) polymerase; Speckle targeted PIP5K1A-regulated poly(A) polymerase; speckle targeted PIP5K1A-regulated poly(A) polymerase; terminal uridylyl transferase 1, U6 snRNA-specific; RNA-binding motif protein 21; RNA-binding protein 21; U6 snRNA-specific terminal uridylyltransferase 1 (EC:2.7.7.52); U6-TUTase]

products gene name :

[TUT1]

other gene names :

[TUT1; TUT1; PAPD2; RBM21; URLC6; TUTase; STARPAP; RBM21; Star-PAP; RNA-binding protein 21; U6-TUTase]

uniprot entry name :

STPAP_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

912

specificity :

The antibody detects endogenous levels of total TUT1 protein.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1.0 mg/ml

storage stability :

Store at -20°C

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Western blotting: 1:500~1:3000. Immunohistochemistry: 1:50~1:100

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen Type: Peptide. Immunogen Description: Synthesized peptide derived from internal of human TUT1.

other info2 :

Target Name: TUT1. SDS-PAGE MW: 95kd

products categories :

Total protein Ab

products description :

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.Ota T., Nat. Genet. 36:40-45(2004).The MGC Project Team, Genome Res. 14:2121-2127(2004).Trippe R.,RNA 12:1494-1504(2006).

ncbi gi num :

226371750

ncbi acc num :

NP_073741.2

ncbi gb acc num :

NM_022830.2

uniprot acc num :

Q9H6E5

ncbi mol weight :

93,847 Da

ncbi summary :

This gene encodes a nucleotidyl transferase that functions as both a terminal uridylyltransferase and a nuclear poly(A) polymerase. The encoded enzyme specifically adds and removes nucleotides from the 3' end of small nuclear RNAs and select mRNAs and may function in controlling gene expression and cell proliferation.[provided by RefSeq, Apr 2009]

uniprot summary :

RBM21: Highly specific terminal uridylyltransferase that exclusively accepts U6 snRNA as substrate. U6 snRNA is unique in that nucleotides are both added to and removed from its 3 -end. U6-TUTase is responsible for a controlled elongation reaction that results in the restoration of the four 3 -terminal UMP-residues found in newly transcribed U6 snRNA. Does not play a role in replication-dependent histone mRNA degradation. Protein type: Nucleolus; EC 2.7.7.52; EC 2.7.7.19; Transferase. Chromosomal Location of Human Ortholog: 11q12.2. Cellular Component: nucleoplasm; intercellular bridge; nucleolus; nuclear speck; mRNA cleavage and polyadenylation specificity factor complex. Molecular Function: polynucleotide adenylyltransferase activity; protein binding; enzyme binding; mRNA 3 -UTR binding; RNA binding; metal ion binding; RNA uridylyltransferase activity; ATP binding. Biological Process: snRNA processing; mRNA polyadenylation; mRNA cleavage

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

255

more info or order :

MyBioSource product webpage