TDP2 Antibody | MyBioSource MBS9409560 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

TDP2 Antibody

catalog :

MBS9409560

quantity :

0.1 mL

price :

255 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Gel: 8%SDS-PAGE Lysates (from left to right): Hela and 293T cell, mouse liver tissue Amount of lysate: 40ug per lane Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000 Exposure time: 6 minutes

image 2 :

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using at dilution 1/40.

product information

catalog number :

MBS9409560

products type :

Antibody

products full name :

TDP2 Antibody

products short name :

[TDP2]

products name syn :

[EAP2; AD022; EAPII; TTRAP; hTDP2; dJ30M3.3]

other names :

[tyrosyl-DNA phosphodiesterase 2; Tyrosyl-DNA phosphodiesterase 2; tyrosyl-DNA phosphodiesterase 2; tyrosyl-DNA phosphodiesterase 2; 5'-tyrosyl-DNA phosphodiesterase; 5'-Tyr-DNA phosphodiesterase; ETS1-associated protein 2; ETS1-associated protein II; EAPII; TRAF and TNF receptor-associated protein; Tyrosyl-RNA phosphodiesterase; VPg unlinkase]

products gene name :

[TDP2]

other gene names :

[TDP2; TDP2; EAP2; AD022; EAPII; TTRAP; hTDP2; dJ30M3.3; EAP2; TTRAP; Tyr-DNA phosphodiesterase 2; hTDP2; 5'-Tyr-DNA phosphodiesterase; EAPII]

uniprot entry name :

TYDP2_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

362

specificity :

The antibody detects endogenous levels of total TDP2 protein.

purity :

Antigen affinity purification.

form :

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

concentration :

2.6 mg/ml

storage stability :

Store at -20 degree C

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Western blotting: 1:200-1:1000. Immunohistochemistry: 1:50-1:200

image1 heading :

Testing Data

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen Type: Peptide. Immunogen Description: Synthetic peptide corresponding to a region derived from internal residues of human tyrosyl-DNA phosphodiesterase 2

other info2 :

Target Name: TDP2

products categories :

Total protein Ab

products description :

This gene encodes a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors.

ncbi gi num :

23510348

ncbi acc num :

NP_057698.2

ncbi gb acc num :

NM_016614.2

uniprot acc num :

O95551

ncbi mol weight :

41kd

ncbi pathways :

CD40/CD40L Signaling Pathway (138061); DNA Repair Pathway (1270350); Double-Strand Break Repair Pathway (1270380); Nonhomologous End-Joining (NHEJ) Pathway (1270395); Processing Of DNA Ends Prior To End Rejoining Pathway (1270396)

ncbi summary :

uniprot summary :

TTRAP: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5 -phosphodiester bond, giving rise to DNA with a free 5 phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5 - phosphoglycolates on protruding 5 ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5 -tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a clean DSB with 5 -phosphate termini that are ready for ligation. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Has also 3 - tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5 - tyrosyl-DNA phosphodiesterase in cells. Also acts as a 5 -tyrosyl- RNA phosphodiesterase following picornavirus infection: its activity is hijacked by picornavirus and acts by specifically cleaving the protein-RNA covalent linkage generated during the viral genomic RNA replication steps of a picornavirus infection, without impairing the integrity of viral RNA. Also acts as an adapter by participating to the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF- beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation. Involved in non- canonical TGF-beta induced signaling routes. May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation. Acts as a regulator of ribosome biogenesis following stress. Belongs to the CCR4/nocturin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.4.-; Nucleolus; Inhibitor. Chromosomal Location of Human Ortholog: 6p22.3-p22.1. Cellular Component: nucleoplasm; PML body; intercellular bridge; cytoplasm; nucleolus; nucleus. Molecular Function: protein binding; nuclease activity; manganese ion binding; magnesium ion binding; transcription corepressor activity; single-stranded DNA binding. Biological Process: cell surface receptor linked signal transduction; double-strand break repair

size1 :

0.1 mL

price1 :

255 USD

more info or order :

MyBioSource product webpage