product summary
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company name :
MyBioSource
product type :
antibody
product name :
MGMT antibody
catalog :
MBS9409314
quantity :
0.1 mL
price :
255 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry
more info or order :
image
image 1 :
Western blot analysis of Hela cell lysate using MGMT antibody.
image 2 :
Immunofluorescence analysis of U20S cell using MGMT antibody. Blue: DAPI for nuclear staining.
product information
catalog number :
MBS9409314
products type :
Antibody
products full name :
MGMT antibody
products short name :
[MGMT]
products name syn :
[MGMT]
other names :
[methylated-DNA--protein-cysteine methyltransferase isoform X2; Methylated-DNA--protein-cysteine methyltransferase; methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase]
products gene name :
[MGMT]
other gene names :
[MGMT; MGMT; MGMT]
uniprot entry name :
MGMT_HUMAN
clonality :
Polyclonal
host :
Rabbit
reactivity :
Human, Mouse, Rat
sequence length :
207
specificity :
The antibody detects endogenous level of total MGMT antibody.
purity :
Antibodies were purified by affinity purification using immunogen.
form :
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
concentration :
1.0 mg/ml
storage stability :
Store at -20 degree C
tested application :
Western Blot (WB), Immunofluorescence (IF)
app notes :
Western blotting: 1:500 - 1:2000. Immunofluorescence: 1:50 - 1:200
image1 heading :
Western Blot (WB)
image2 heading :
Immunofluorescence (IF)
other info1 :
Immunogen Type: Recombinant Protein. Immunogen Description: Recombinant protein of human MGMT.
other info2 :
Target Name: MGMT. Acession Number: P16455NCBI. Gene ID: 4255
products categories :
Total protein Ab
products description :
MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O(6) position of guanine, restoring guanine to its normal form without causing DNA breaks (1). MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents (2,3). It is ubiquitously expressed in normal human tissues (4) and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy (5).
ncbi gi num :
530394832
ncbi acc num :
XP_005252739.1
ncbi gb acc num :
XM_005252682.2
uniprot acc num :
P16455
ncbi mol weight :
22kd
ncbi pathways :
DNA Damage Reversal Pathway (1270375); DNA Repair Pathway (1270350); MGMT-mediated DNA Damage Reversal Pathway (1270376)
uniprot summary :
MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family. Protein type: Nuclear receptor co-regulator; Methyltransferase; DNA repair, damage; EC 2.1.1.63; Apoptosis. Chromosomal Location of Human Ortholog: 10q26. Cellular Component: nucleoplasm; membrane; nucleus. Molecular Function: methyltransferase activity; methylated-DNA-[protein]-cysteine S-methyltransferase activity; DNA binding; damaged DNA binding; calcium ion binding; DNA-methyltransferase activity. Biological Process: response to drug; response to ethanol; positive regulation of DNA repair; DNA dealkylation; response to toxin; response to folic acid; DNA methylation; regulation of caspase activity; DNA repair; DNA ligation
size1 :
0.1 mL
price1 :
255 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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