Mouse Programmed cell death 1 ligand 1, CD274 ELISA Kit | MyBioSource MBS940928 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Programmed cell death 1 ligand 1, CD274 ELISA Kit

catalog :

MBS940928

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS940928

products type :

ELISA Kit

products full name :

Mouse Programmed cell death 1 ligand 1, CD274 ELISA Kit

products short name :

CD274 molecule

products name syn :

Mouse Programmed cell death 1 ligand 1 (CD274) ELISA kit; B7-H; B7H1; MGC142294; MGC142296; PD-L1; PDCD1L1; PDCD1LG1; PDL1; CD274 antigen; programmed cell death 1 ligand 1; CD274 molecule

other names :

programmed cell death 1 ligand 1; Programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1; CD274 antigen; B7 homolog 1; B7-H1; CD_antigen: CD274

products gene name :

CD274

other gene names :

Cd274; Cd274; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik; B7h1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1

uniprot entry name :

PD1L1_MOUSE

reactivity :

Mouse

sequence length :

290

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse CD274. No significant cross-reactivity or interference between mouse CD274 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: The minimum detectable dose of mouse CD274 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD274 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD274 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD274 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD274 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

11230798

ncbi acc num :

NP_068693.1

ncbi gb acc num :

NM_021893.3

uniprot acc num :

Q9EP73

ncbi mol weight :

32,780 Da

ncbi pathways :

Adaptive Immune System Pathway (575096); Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); Costimulation By The CD28 Family Pathway (575102); Immune System Pathway (575095); PD-1 Signaling Pathway (575107)

uniprot summary :

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Cellular Component: cell surface; membrane; plasma membrane; integral to membrane; external side of plasma membrane. Molecular Function: protein binding. Biological Process: cell surface receptor linked signal transduction; negative regulation of T cell proliferation; negative regulation of interleukin-10 production; negative regulation of interferon-gamma production; negative regulation of activated T cell proliferation; T cell costimulation; immune response; positive regulation of T cell proliferation; signal transduction; positive regulation of cell migration

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage