catalog number :
MBS940912
products type :
ELISA Kit
products full name :
Mouse Chymotrypsin-C, CTRC ELISA Kit
products short name :
chymotrypsin C (caldecrin)
products name syn :
Mouse Chymotrypsin-C (CTRC) ELISA kit; CLCR; ELA4; caldecrin; chymotrypsin C; elastase 4; elastase IV; serum calcium decreasing factor; chymotrypsin C (caldecrin)
other names :
chymotrypsin-C; Chymotrypsin-C; chymotrypsin-C; chymotrypsin C (caldecrin)
products gene name :
CTRC
other gene names :
Ctrc; Ctrc; 1810044E12Rik
uniprot entry name :
CTRC_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse CTRC. No significant cross-reactivity or interference between mouse CTRC and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 ng/ml -2000 ng/ml. Sensitivity: The minimum detectable dose of mouse CTRC is typically less than 7.81 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CTRC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTRC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTRC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTRC bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001029047.1
ncbi gb acc num :
NM_001033875.2
ncbi mol weight :
29,470 Da
uniprot summary :
CTRC: Has chymotrypsin-type protease activity and hypocalcemic activity. Genetic variations in CTRC can predispose to chronic pancreatitis by diminishing its protective trypsin-degrading activity. Belongs to the peptidase S1 family. Elastase subfamily. Protein type: EC 3.4.21.2; Protease. Cellular Component: cell. Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity. Biological Process: cellular calcium ion homeostasis; proteolysis
size4 :
10x96-Strip-Wells