catalog number :
MBS940905
products type :
ELISA Kit
products full name :
Human Protein FAM132B, FAM132B ELISA Kit
products short name :
family with sequence similarity 132, member B
products name syn :
Human Protein FAM132B (FAM132B) ELISA kit; FLJ37034; hypothetical protein LOC151176; family with sequence similarity 132; member B
other names :
protein FAM132B; Erythroferrone; protein FAM132B; myonectin; erythroferrone; complement C1q tumor necrosis factor-related protein 15; family with sequence similarity 132, member B; Complement C1q tumor necrosis factor-related protein 15; Myonectin
products gene name :
FAM132B
other gene names :
FAM132B; FAM132B; CTRP15; C1QTNF15; C1QTNF15; CTRP15; ERFE
uniprot entry name :
ERFE_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human FAM132B. No significant cross-reactivity or interference between human FAM132B and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates, cell lysates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 3.9 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FAM132B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FAM132B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAM132B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAM132B bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001278761.1
ncbi gb acc num :
NM_001291832.1
ncbi mol weight :
37,279 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
FAM132B: Belongs to the FAM132 family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2q37.3. Cellular Component: extracellular space; extracellular region. Molecular Function: protein homodimerization activity; hormone activity. Biological Process: cellular iron ion homeostasis; regulation of fatty acid metabolic process
size4 :
10x96-Strip-Wells