antibody

NFkappaB-p65 (Acetyl-Lys314/Lys315) Polyclonal Antibody

MBS9408791

0.05 mL

200 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, enzyme immunoassay

Antibody

NFkappaB-p65 (Acetyl-Lys314/Lys315) Polyclonal Antibody

[NFkappaB-p65]

[NFkB-p65 (Acetyl-Lys314/Lys315) Polyclonal Antibody; RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3]

[transcription factor p65 isoform 2; Transcription factor p65; transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3]

[NFkappaB-p65]

[RELA; RELA; p65; NFKB3; NFKB3]

TF65_HUMAN

Polyclonal

Rabbit

Human, Mouse, Rat

548

Acetyl-NFkB-p65 (K314/K315) Polyclonal AntibodySynthesized peptide derived from the human NFkB-p65 around the acetylation site of K314/K315.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

1 mg/ml

Store at -20°C/1 year

Western Blot (WB), ELISA (EIA)

Western blotting: 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications

Western Blot (WB)

Western Blot (WB)

Immunogen Type: peptide. Immunogen Description: Synthesized peptide derived from the human NFkB-p65 around the acetylation site of K314/K315. SDS-PAGE: 60kD

Modification: Acetyl-Lys314/Lys315. Target Species: Human. Target Name: NFkB-p65

Acetyl-specific Ab

223468681

NP_001138610.1

NM_001145138.1

Q04206

AGE/RAGE Pathway (698754); Activated TLR4 Signalling Pathway (1269236); Activation Of NF-kappaB In B Cells Pathway (1269186); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (1269171); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191)

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified. Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription factor. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; identical protein binding; transcription activator binding; protein N-terminus binding; protein kinase binding; transcription factor binding; NF-kappaB binding; protein binding; DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; protein complex binding; chromatin binding; phosphate binding; transcription factor activity. Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of insulin receptor signaling pathway; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; hair follicle development; toll-like receptor 4 signaling pathway; defense response to virus; response to drug; membrane protein intracellular domain proteolysis; positive regulation of I-kappaB kinase/NF-kappaB cascade; acetaldehyde metabolic process; response to amino acid stimulus; toll-like receptor 2 signaling pathway; organ morphogenesis; positive regulation of chondrocyte differentiation; response to mechanical stimulus; response to muramyl dipeptide; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; response to progesterone stimulus; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; transcription from RNA polymerase II promoter; response to cAMP; response to morphine; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 10 signaling pathway; response to insulin stimulus; response to organic substance; positive regulation of cell proliferation; positive regulation of interferon type I production; inflammatory response; aging; positive regulation of Schwann cell differentiation; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; liver development; response to UV-B; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-12 biosynthetic process; regulation of inflammatory response; toll-like receptor signaling pathway; cellular defense response; innate immune response; response to cobalamin

0.05 mL

200 USD

0.1 mL

255