PTPN22 antibody | MyBioSource MBS9408681 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PTPN22 antibody

catalog :

MBS9408681

quantity :

0.1 mL

price :

255 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of Jurkat cell lines, using PTPN22 antibody.

product information

catalog number :

MBS9408681

products type :

Antibody

products full name :

PTPN22 antibody

products short name :

[PTPN22]

products name syn :

[LYP; LYP1; LYP2; PEP; PTPN8; PTPN22]

other names :

[tyrosine-protein phosphatase non-receptor type 22 isoform 3; Tyrosine-protein phosphatase non-receptor type 22; tyrosine-protein phosphatase non-receptor type 22; protein tyrosine phosphatase, non-receptor type 22 (lymphoid); Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; LyP; PEST-domain phosphatase; PEP]

products gene name :

[PTPN22]

other gene names :

[PTPN22; PTPN22; LYP; PEP; LYP1; LYP2; PTPN8; PTPN8; LyP; PEP]

uniprot entry name :

PTN22_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

779

specificity :

The antibody detects endogenous level of total PTPN22 antibody.

purity :

Antibodies were purified by affinity purification using immunogen.

form :

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1.0 mg/ml

storage stability :

Store at -20 degree C

tested application :

Western Blot (WB)

app notes :

Western blotting: 1:500 - 1:2000

image1 heading :

Western Blot (WB)

other info1 :

Immunogen Type: Recombinant Protein. Immunogen Description: Recombinant protein of human PTPN22.

other info2 :

Target Name: PTPN22

products categories :

Total protein Ab

products description :

PTPN22 (Lyp/PEP) is a cytoplasmic phosphatase expressed by hematopoietic cells (1,2). PTPN22 associates with the tyrosine kinase Csk to inhibit T cell receptor signaling through inactivation of Src kinases (3,4). Csk phosphorylates Src kinases on an inhibitory tyrosine, while PTPN22 dephosphorylates an activating site (4). PTPN22(-/-) mice have higher levels of activated Lck than wild-type, resulting in greater T cell expansion and increased serum antibody levels (5). Research studies have shown that a single-nucleotide polymorphism, 1858T of the PTPN22 gene which encodes the amino acid substitution R620W, confers increased risk for multiple autoimmune diseases including type I diabetes, rheumatoid arthritis, systemic lupus erythematosus, and Graves disease (6-9). Interestingly, although the R620W substitution disrupts the interaction between Csk and PTPN22, it is actually a gain-of-function mutation resulting in increased phosphatase activity (6,10,11). Recent evidence suggests that the autoimmune phenotype associated with the R620W variant is the result of increased calpain-mediated degradation and decreased protein levels of PTPN22 (12).

ncbi gi num :

301171669

ncbi acc num :

NP_001180360.1

ncbi gb acc num :

NM_001193431.2

uniprot acc num :

Q9Y2R2

ncbi mol weight :

SDS-PAGE: 92kd

ncbi summary :

This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Mar 2009]

uniprot summary :

PTPN22: Seems to act on Cbl. May play a role in regulating the function of Cbl and its associated protein kinases. Acts as negative regulator of T-cell receptor (TCR) signaling. Dephosphorylates and inactivates the SRC family kinases. Interacts with CSK. Interacts with LPXN. Predominantly expressed in lymphoid tissues and cells. Isoform 1 is expressed in thymocytes and both mature B and T-cells. Down-regulated by phosphorylation. Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1p13.2. Cellular Component: internal side of plasma membrane; perinuclear region of cytoplasm; cytoplasm; nucleus. Molecular Function: protein binding; protein tyrosine phosphatase activity; kinase binding; SH3 domain binding. Biological Process: regulation of B cell receptor signaling pathway; regulation of natural killer cell proliferation; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation; negative regulation of T cell activation; regulation of innate immune response; T cell differentiation. Disease: Diabetes Mellitus, Insulin-dependent; Systemic Lupus Erythematosus; Rheumatoid Arthritis

size1 :

0.1 mL

price1 :

255 USD

more info or order :

MyBioSource product webpage