PIKFYVE antibody | MyBioSource MBS9407908 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PIKFYVE antibody

catalog :

MBS9407908

quantity :

0.1 mL

price :

255 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of Mouse brain cell line, using PIKFYVE antibody.

image 2 :

Immunofluorescence analysis of U2OS cell using PIP5K3 antibody. Blue: DAPI for nuclear staining.

product information

catalog number :

MBS9407908

products type :

Antibody

products full name :

PIKFYVE antibody

products short name :

[PIKFYVE]

products name syn :

[CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29]

other names :

[1-phosphatidylinositol 3-phosphate 5-kinase isoform 4; 1-phosphatidylinositol 3-phosphate 5-kinase; 1-phosphatidylinositol 3-phosphate 5-kinase; phosphoinositide kinase, FYVE finger containing; FYVE finger-containing phosphoinositide kinase; PIKfyve; Phosphatidylinositol 3-phosphate 5-kinase type III; PIPkin-III; Type III PIP kinase]

products gene name :

[PIKFYVE]

other gene names :

[PIKFYVE; PIKFYVE; CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29; KIAA0981; PIP5K3; Phosphatidylinositol 3-phosphate 5-kinase; PIPkin-III; Type III PIP kinase]

uniprot entry name :

FYV1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

548

specificity :

The antibody detects endogenous level of total PIKFYVE antibody.

purity :

Antibodies were purified by affinity purification using immunogen.

form :

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1.0 mg/ml

storage stability :

Store at -20°C

tested application :

Western Blot (WB), Immunofluorescence (IF)

app notes :

Western blotting: 1:500 - 1:2000. Immunofluorescence: 1:50 - 1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Immunogen Type: Recombinant Protein. Immunogen Description: Recombinant protein of human PIKFYVE.

other info2 :

Target Name: PIKFYVE. SDS-PAGE MW: 273 kD

products categories :

Total protein Ab

products description :

Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

ncbi gi num :

295789162

ncbi acc num :

NP_001171471.1

ncbi gb acc num :

NM_001178000.1

uniprot acc num :

Q9Y2I7

ncbi mol weight :

237kd

ncbi pathways :

3-phosphoinositide Biosynthesis Pathway (545314); 3-phosphoinositide Biosynthesis Pathway (138600); Inositol Phosphate Metabolism Pathway (82987); Inositol Phosphate Metabolism Pathway (362); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001); PI Metabolism Pathway (1270073); Phagosome Pathway (153910); Phagosome Pathway (153859); Phosphatidylinositol Signaling System Pathway (83052)

ncbi summary :

uniprot summary :

PIKFYVE: The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo- inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes. Defects in PIKFYVE are the cause of corneal fleck dystrophy (CFD). CFD is an autosomal dominant disorder of the cornea characterized by numerous small white flecks scattered in all levels of the stroma. Although CFD may occasionally cause mild photophobia, patients are typically asymptomatic and have normal vision. 4 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.7.1.150; Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate; Kinase, lipid. Chromosomal Location of Human Ortholog: 2q34. Cellular Component: Golgi membrane; perinuclear region of cytoplasm; late endosome membrane; early endosome membrane; endosome membrane; intercellular junction; cytoplasmic vesicle; cytosol; lipid raft. Molecular Function: protein binding; 1-phosphatidylinositol-3-phosphate 5-kinase activity; 1-phosphatidylinositol-4-phosphate 5-kinase activity; phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity; metal ion binding; ATP binding. Biological Process: myelin formation; receptor-mediated endocytosis; cellular protein metabolic process; phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; retrograde transport, endosome to Golgi. Disease: Corneal Dystrophy, Fleck

size1 :

0.1 mL

price1 :

255 USD

more info or order :

MyBioSource product webpage