Human Cytochrome P450 19A1, CYP19A1 ELISA Kit | MyBioSource MBS940663 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Cytochrome P450 19A1, CYP19A1 ELISA Kit

catalog :

MBS940663

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS940663

products type :

ELISA Kit

products full name :

Human Cytochrome P450 19A1, CYP19A1 ELISA Kit

products short name :

cytochrome P450, family 19, subfamily A, polypeptide 1

products name syn :

Human Cytochrome P450 19A1 (CYP19A1) ELISA kit; ARO; ARO1; CPV1; CYAR; CYP19; MGC104309; P-450AROM; OTTHUMP00000162543; aromatase; cytochrome P450; subfamily XIX (aromatization of androgens) ; estrogen synthetase; flavoprotein-linked monooxygenase; mic; cytochrome P450; family 19; subfamily A; polypeptide 1

other names :

aromatase; Aromatase; aromatase; estrogen synthase; estrogen synthetase; cytochrome P-450AROM; cytochrome P450 19A1; microsomal monooxygenase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily XIX (aromatization of androgens); cytochrome P450, family 19, subfamily A, polypeptide 1; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase

products gene name :

CYP19A1

other gene names :

CYP19A1; CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM; ARO1; CYAR; CYP19

uniprot entry name :

CP19A_HUMAN

reactivity :

Human

sequence length :

503

specificity :

This assay has high sensitivity and excellent specificity for detection of human CYP19A1. No significant cross-reactivity or interference between human CYP19A1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 26.7 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of human CYP19A1 is typically less than 6.7 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human CYP19A1 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP19A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for CYP19A1. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP19A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

13904858

ncbi acc num :

NP_000094.2

ncbi gb acc num :

NM_000103.3

uniprot acc num :

P11511

ncbi mol weight :

57,883 Da

ncbi pathways :

Biological Oxidations Pathway (105698); C19/C18-Steroid Hormone Biosynthesis, Pregnenolone = Androstenedione = Estrone Pathway (413383); C19/C18-Steroid Hormone Biosynthesis, Pregnenolone = Androstenedione = Estrone Pathway (468268); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Endogenous Sterols Pathway (105701); Estrogen Biosynthesis Pathway (106155); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamins A And D Pathway (106150); Ovarian Infertility Genes Pathway (198801)

ncbi summary :

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

uniprot summary :

CYP19A1: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Belongs to the cytochrome P450 family. Protein type: EC 1.14.14.14; Oxidoreductase; Lipid Metabolism - androgen and estrogen. Chromosomal Location of Human Ortholog: 15q21.1. Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum. Molecular Function: electron carrier activity; iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding. Biological Process: steroid metabolic process; xenobiotic metabolic process; androgen metabolic process; sterol metabolic process; estrogen biosynthetic process; steroid biosynthetic process. Disease: Aromatase Deficiency; Aromatase Excess Syndrome

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage