CHAT Antibody | MyBioSource MBS9406559 product information

product summary

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company name :

MyBioSource

product type :

antibody

product name :

CHAT Antibody

catalog :

MBS9406559

quantity :

0.1 mL

price :

255 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using RAD51 antibody.

image 2 :

Immunofluorescence analysis of MCF-7 cell using CHAT antibody.

product information

catalog number :

MBS9406559

products type :

Antibody

products full name :

CHAT Antibody

products short name :

[CHAT]

products name syn :

[CMS1A; CMS1A2; CHOACTASE]

other names :

[choline O-acetyltransferase isoform 1; Choline O-acetyltransferase; choline O-acetyltransferase; choline O-acetyltransferase]

products gene name :

[CHAT]

other gene names :

[CHAT; CHAT; CMS6; CMS1A; CMS1A2; CHOACTASE; CHOACTase; ChAT; Choline acetylase]

uniprot entry name :

CLAT_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

630

specificity :

The antibody detects endogenous level of total CHAT protein.

purity :

Antibodies were purified by affinity purification using immunogen.

form :

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1.0 mg/ml

storage stability :

Store at -20°C

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

Western blotting: 1:500 - 1:2000. Immunohistochemistry: 1:50 - 1:200. Immunofluorescence: 1:50 - 1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Immunogen Type: Recombinant Protein. Immunogen Description: Recombinant protein of human CHAT.

other info2 :

Target Name: CHAT. SDS-PAGE MW: 83 kD

products categories :

Total protein Ab

products description :

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.

ncbi gi num :

218931221

ncbi acc num :

NP_001136401.1

ncbi gb acc num :

NM_001142929.1

uniprot acc num :

P28329

ncbi pathways :

Acetylcholine Neurotransmitter Release Cycle Pathway (1268773); Acetylcholine Synthesis Pathway (198820); Biogenic Amine Synthesis Pathway (198793); Cholinergic Synapse Pathway (217716); Glycerophospholipid Biosynthesis Pathway (1270054); Glycerophospholipid Metabolism Pathway (82989); Glycerophospholipid Metabolism Pathway (364); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001); Neuronal System Pathway (1268763)

ncbi summary :

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]

uniprot summary :

CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described. Protein type: EC 2.3.1.6; Lipid Metabolism - glycerophospholipid; Acetyltransferase. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: mitochondrion; cell soma; axon; cytoplasm; cytosol; nucleus. Molecular Function: choline O-acetyltransferase activity. Biological Process: synaptic transmission; rhythmic excitation; rhythmic behavior; muscle development; neurotransmitter secretion; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylcholine biosynthetic process; dendrite development; neuromuscular synaptic transmission; establishment of synaptic specificity at neuromuscular junction; adult walking behavior; neurotransmitter biosynthetic process. Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea

size1 :

0.1 mL

price1 :

255 USD

more info or order :

MyBioSource product webpage