TPM2 Antibody | MyBioSource MBS9406393 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

TPM2 Antibody

catalog :

MBS9406393

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Gel: 10%SDS-PAGE . Lysate: 40 ug Human cervical cancer tissue lysate. Primary antibody: 1/750 dilution. Secondary antibody: Goat anti Rabbit IgG - H&L (HRP). at 1/10000 dilution . Exposure time: 2 minutes

image 2 :

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using MBS9406393 (TPM2 Antibody) at dilution 1/35, on the right is treated with the synthetic peptide.

product information

catalog number :

MBS9406393

products type :

Antibody

products full name :

TPM2 Antibody

products short name :

[TPM2]

products name syn :

[tropomyosin 2 (beta), DA1, DA2B, NEM4, TMSB, AMCD1]

other names :

[tropomyosin beta chain isoform Tpm2.1sm/cy; Tropomyosin beta chain; tropomyosin beta chain; tropomyosin 2 (beta); Beta-tropomyosin; Tropomyosin-2]

products gene name :

[TPM2]

other gene names :

[TPM2; TPM2; DA1; DA2B; NEM4; TMSB; AMCD1; HEL-S-273; TMSB]

uniprot entry name :

TPM2_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human

sequence length :

284

specificity :

The antibody detects endogenous level of total TPM2 protein.

form :

Supplied at 1.5mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.

storage stability :

Store at -20°C/1 year

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

ELISA: 1:1000-1:5000. Western blotting: 1:2000-1:5000. Immunohistochemistry: 1:50-1:200

image1 heading :

Testing Data

image2 heading :

Immunohistochemistry (IHC)

products categories :

Total protein Ab

products description :

Background: This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. TPM2 has been shown to interact with RRAD, PDLIM7 and TPM1.

ncbi gi num :

47519616

ncbi acc num :

NP_998839

ncbi gb acc num :

NM_213674.1

ncbi pathways :

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Cardiac Muscle Contraction Pathway (93344); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (1269868); Smooth Muscle Contraction Pathway (1269870)

ncbi summary :

This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

uniprot summary :

TPM2: Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization. Defects in TPM2 are the cause of nemaline myopathy type 4 (NEM4). A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 4 presents from infancy to childhood with hypotonia and moderate-to-severe proximal weakness with minimal or no progression. Major motor milestones are delayed but independent ambulation is usually achieved, although a wheelchair may be needed in later life. Defects in TPM2 are the cause of distal arthrogryposis type 1A (DA1A). A form of distal arthrogryposis, a disease characterized by congenital joint flexures or contractures that mainly involve the distal parts of the limbs, and affect two or more different body areas in the absence of a primary neurological or muscle disease. Distal arthrogryposis type 1 is characterized largely by camptodactyly and clubfoot. Hypoplasia and/or absence of some interphalangeal creases is common. The shoulders and hips are less frequently affected. Belongs to the tropomyosin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Motor. Chromosomal Location of Human Ortholog: 9p13. Cellular Component: cytosol; muscle thin filament tropomyosin. Molecular Function: structural constituent of muscle; actin binding. Biological Process: regulation of ATPase activity; muscle contraction; muscle filament sliding. Disease: Nemaline Myopathy 4; Arthrogryposis, Distal, Type 1a; Myopathy, Congenital, With Fiber-type Disproportion; Arthrogryposis, Distal, Type 2b

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

255

more info or order :

MyBioSource product webpage